OBJECTIVE: To study the distribution of sliver in plasma and tissues of burnt rats with Nano-silver cream. METHODS: Deep IT degree scald model of 5% total body surface area (5 cm×5 cm) of rats was established and randomly divided into Nano-silver cream group, Flamazine cream group and base control group with 8 rats in each group. They were applied cream to injured infection site coating with 0.1-0.2 cm, once a day for consecutive 30 d. Rats were sacrificed 24 h after drug withdrawal. The contents of sliver in plasma, heart, liver, spleen and kidney were determined by ICP-MS. The condition of determination was as follows: radio-frequency power 1 250 W, impulse voltage 1 200 V, flow velocity of atomizing gas 0.80 L/min, flow velocity of plasma gas 15 L/min. RESULTS: The linear range of sliver was 0.01-10.00 μg/ml (r=0.999 9) with recoveries of (93.2 ± 3.3)%-(112.5 ± 1.0)% (RSD<3.5% , n=8). Compared with base control group, the contents of sliver in plasma, heart, liver, spleen and kidney increased significantly in Flamazine cream group (P<0.05 or P<0.01), while the contents of sliver in only spleen increased significantly in Nano-silver cream group (P<0.05). CONCLUSIONS: Results show that no significant accumulations of Nano-silver cream in plasma and tissue except spleen of rats.%目的:研究纳米银乳膏在烧伤模型大鼠血浆及组织中银的分布.方法:建立大鼠5％体表总面积(5 cm×5 cm)深Ⅱ度蒸气烧伤模型,分为纳米银乳膏组、磺胺嘧啶银乳膏组、基质对照组,每组8只,分别于受伤感染处涂药0.1～0.2 cm厚,每日1次,连续30d,停药24h后处死大鼠,利用电感耦合等离子体质谱仪测定各组大鼠血浆和心、肝、脾、肾组织中的银含量.仪器条件:射频功率为1 250 W,脉冲电压为1 200 V,雾化气流速为0.80 L/min,等离子气流速为15 L/min.结果:银检测质量浓度的线性范围为0.01～10.00 μg/ml(r=0.999 9),加样回收率为(93.2±3.3)％～(112.5±1.0)％,RSD＜3.5％(n=8).与基质对照组比较,磺胺嘧啶银乳膏组大鼠的血浆和心、肝、脾、肾组织中银含量均明显增加(P＜0.05或P＜0.01),而纳米银乳膏组大鼠仅脾组织中银含量明显增加(P＜0.05).结论:纳米银乳膏在大鼠血浆和除脾以外的各组织中无明显蓄积.
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