石柑子药材的HPLC指纹图谱研究

摘要

目的：建立石柑子药材的高效液相色谱（HPLC）指纹图谱。方法：采用HPLC法。色谱柱为Thermo Hypersil ODS-2，流动相为甲醇-0.4％磷酸溶液（梯度洗脱），流速为1.0 ml/min，检测波长为300 nm，柱温为30℃，进样量为10μl。以阿魏酰酪胺色谱峰为参照峰，测定24批药材的HPLC图谱，采用《中药色谱指纹图谱相似度评价系统》（2004A版）进行共有峰鉴定和相似度评价。结果：24批石柑子药材有16个共有峰，其中21批药材的相似度＞0.8。结论：该研究所建指纹图谱专属性强、结果稳定，可为石柑子药材的质量控制提供依据。%OBJECTIVE:To establish the HPLC fingerprint of Pothos chinensis. METHODS:HPLC was performed on the col-umn of Thermo Hypersil ODS-2 with mobile phase of methanol-0.4% phosphoric acid solution(gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 300 nm,column temperature was 30 ℃,and injection volume was 10 μl. Using ferulic acid tyramine peak as reference,HPLC spectrum of 24 batches of medicinal herbs was determined,and TCM Chromatographic Finger-print Similarity Evaluation System(2004 edition)was adopted for common peak identification and similarity evaluation. RESULTS:There were 16 common peaks totally in the 24 batches ,similarity of the 21 batches of medicinal materials were higher than 0.8. CONCLUSIONS:The established fingerprint is specific,stable,and can provide basis for quality control of P. chinensis.