HPLC法同时测定不同产地羊蹄药材中大黄素、大黄酚、大黄素甲醚的含量

摘要

OBJECTIVE:To establish a method for contents determination of emodin,chrysophanol and physcion in Rumex ja-ponicus,and compared the differences of components in medicinal materials from different producing areas. METHODS:HPLC was performed on the column of Agilent C18 with mobile phase of methanal-0.1% phosphoric acid solution (gradient elution) at a flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 35 ℃,and injection volume was 20 μl. RE-SULTS:The linear range was 2.04-24.48 μg/ml for emodin (r=0.999 7),2.00-24.00 μg/ml for chrysophanol (r=0.999 5) and 1.02-12.24 μg/ml for physcion(r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recover-ies were 97.09%-102.65%(RSD=1.79%,n=9),97.10%-100.31%(RSD=0.97%,n=9)and 95.45%-100.18%(RSD=1.45%, n=9). CONCLUSIONS:The method is simple with high precision,stability and reproducibility,and can be used for the simulta-neous determination of emodin,chrysophanol and physcion in R. japonicas.There are obvious differences in medicinal materials from different producing areas.%目的：建立同时测定羊蹄药材中大黄素、大黄酚、大黄素甲醚含量的方法，并比较不同产地药材各成分含量差异。方法：采用高效液相色谱法。色谱柱为Agilent C18，流动相为甲醇-0.1％磷酸溶液（梯度洗脱），流速为1.0 ml/min，检测波长为254 nm，柱温为35℃，进样量为20μl。结果：大黄素、大黄酚、大黄素甲醚检测质量浓度线性范围分别为2.04～24.48μg/ml（r＝0.9997）、2.00～24.00μg/ml（r＝0.9995）、1.02～12.24μg/ml（r＝0.9998）；精密度、稳定性、重复性试验的RSD＜2.0％；加样回收率分别为97.09％～102.65％（RSD＝1.79％，n＝9）、97.10％～100.31％（RSD＝0.97％，n＝9）、95.45％～100.18％（RSD＝1.45％，n＝9）。结论：该方法检测简便，精密度、稳定性、重复性好，可用于羊蹄药材中大黄素、大黄酚、大黄素甲醚含量的同时测定；不同产地药材中各成分含量差异显著。