新疆阿魏乙酸乙酯部位对小鼠S180实体瘤的抑制作用

摘要

OBJECTIVE:To study the inhibitory effect of ethyl acetate part from Ferula sinkiangensis on S180 solid tumor in mice.METHODS:60 mice were randomly divided into normal control group,tumor model group,ethyl acetate part of F. sinkian-gensis low-dose,middle-dose and high-dose groups(1.0,2.0,3.0 g/kg,ig),cisplatin control group(positive drug,5 mg/kg,iv), with 10 mice in each group. Except for normal control group,other groups were given mice ascites infected by S180 solid tumor to induce S180 solid tumor model. The next day after modeling,those groups were given relevant medicine,and normal control group and tumor model group were given constant volume of normal saline,once a day,for consecutive 10 days. Body weight,organ (spleen,thymus)index,the change of tumor volume,volume and weight of solid tumor,inhibitory rate of tumor and pathological change were observed in each group. ELISA method was used to determined the serum levels of IL-2,IFN-γ and TNF-α. RE-SULTS:Compared with normal control group,spleen index and TNF-α of tumor model group were increased significantly,while IL-2 and IFN-γ were decreased significantly (P<0.05);dysplastic cells of tumor tissue appeared lumpy,accompanied by a num-ber of ischemic necrosis of dysplastic cells. Compared with tumor model group,body weight,spleen index,thymus index and vol-ume and weight of solid tumor in cisplatin control group were decreased significantly,while IL-2 and TNF-α levels were increased significantly. The spleen index and TNF-α level of ethyl acetate part of F. sinkiangensis groups were increased significantly,while the volume and weight of solid tumor were decreased significantly(P<0.05). The tumor tissue was inhibited to different extents, especially in cisplatin control group;the number of tumor cells was decreased,accompanied by small number of cellular necrosis and infiltration. Inhibitory rates of cisplatin control group,ethyl acetate part of F. sinkiangensis groups were (73.26 ± 4.55)%, (40.34±3.45)%,(50.86±9.34)%,(58.20±3.70)%,respectively. CONCLUSIONS:ethyl acetate part of F. sinkiangensis can in-hibit S180 solid tumor in mice.%目的：研究新疆阿魏药材乙酸乙酯部位对小鼠S180实体瘤的抑制作用。方法：将60只小鼠随机分为正常对照组，肿瘤模型组，阿魏乙酸乙酯部位低、中、高剂量组（1.0、2.0、3.0 g/kg，ig），顺铂对照组（阳性药物，5 mg/kg，iv），每组10只。除正常对照组外，其余各组小鼠接种经S180实体瘤侵染的小鼠的腹水以复制S180实体瘤动物模型。造模次日，各给药组小鼠给予相应药物，正常对照组、肿瘤模型组小鼠ig等体积生理盐水，每天1次，连续10 d。观察并测定各组小鼠体质量、脏器（脾、胸腺）指数、肿瘤体积变化趋势、实体瘤块体积和质量、肿瘤抑制率和病理变化；酶联免疫吸附法（ELISA）测定小鼠血清白细胞介素2（IL-2）、干扰素γ（IFN-γ）、肿瘤坏死因子α（TNF-α）水平。结果：与正常对照组比较，肿瘤模型组小鼠脾指数、TNF-α水平显著升高，IL-2、IFN-γ水平显著降低（P＜0.05）；肿瘤组织异型细胞呈团块状分布，伴有一定数量的缺血性坏死。与肿瘤模型组比较，顺铂对照组小鼠体质量、脾指数、胸腺指数、实体瘤块体积和质量显著降低，IL-2、TNF-α水平显著升高；阿魏乙酸乙酯部位各剂量组小鼠脾指数、TNF-α水平显著升高，实体瘤块体积和质量显著降低（P＜0.05）；病理观察肿瘤组织受到不同程度抑制，且顺铂对照组抑制程度最为显著，肿瘤细胞数量减少，伴有少量坏死和细胞浸润；顺铂对照组和阿魏乙酸乙酯部位低、中、高剂量组小鼠的肿瘤抑制率分别为（73.26±4.55）％、（40.34±3.45）％、（50.86±9.34）％、（58.20±3.70）％。结论：阿魏药材乙酸乙酯部位对小鼠S180实体瘤有抑制作用。