目的：建立同时测定壮药冰糖草中野黄芩苷、蒙花苷含量的方法。方法：采用高效液相色谱法。色谱柱为Phenomenex C18，流动相为乙腈-0.05％磷酸（梯度洗脱），流速为1.0 ml/min，检测波长为330 nm，柱温30℃，进样量为10μl。结果：野黄芩苷、蒙花苷检测质量浓度线性范围分别为0.0158～0.3168 mg/ml（r＝0.9999）、0.0005～0.0104 mg/ml（r＝0.9997）；精密度、稳定性、重复性试验的RSD＜3％；加样回收率分别为99.56％～102.38％（RSD＝1.07％，n＝6）、95.14％～98.29％（RSD＝1.24％，n＝6）。结论：该方法操作简便，稳定性、重复性好，可用于冰糖草中野黄芩苷、蒙花苷含量的同时测定。%OBJECTIVE:To establish a method for the simultaneous determination of scutellarin and linarin in Scoparia dulcis. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase of acetonitrile-0.05% phosphoric acid (gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 330 nm,column temperature was 30 ℃,and the in-jection volume was 10 μl. RESULTS:The linear range was 0.015 8-0.316 8 mg/ml for scutellarin(r=0.999 9)and 0.000 5-0.010 4 mg/ml for linarin (r=0.999 7);RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 99.56%-102.38%(RSD=1.07%,n=6)and 95.14%-98.29%(RSD=1.24%,n=6),respectively. CONCLUSIONS:The method is simple with good stability and reprodicibility,and can be used for the simultaneous determination of scutellarin and linarin in S. du-lcis.
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