HPLC法测定广升麻植株中不同部位蜕皮甾酮的含量

摘要

目的：建立测定广升麻植株中不同部位蜕皮甾酮含量的方法。方法：采用高效液相色谱法。色谱柱为ZORBAX Eclipse XDB-C18，流动相为甲醇-水（46∶54，V/V），流速为1.0 ml/min，检测波长为248 nm，柱温为30℃，进样量为10μl。比较广升麻植株不同部位中蜕皮甾酮的含量。结果：蜕皮甾酮的检测进样量线性范围为0.12～1.21μg；精密度、稳定性、重复性试验的RSD＜1.5％；加样回收率为98.4％～101.9％，RSD＝1.64（n＝6）。广升麻植株根中的蜕皮甾酮含量最高，其次是块茎，茎和叶的含量较低；种子中则不含蜕皮甾酮成分。结论：该方法简便快速、准确性和重复性好，适用于测定广升麻植株中不同部位蜕皮甾酮的含量；且可以将广升麻的药用部位由原来的根扩展为根、花、块茎以及块茎上的芽。%OBJECTIVE:To establish a method for the content determination of ecdysterone in different parts of Radix serratu-lae. METHODS:HPLC was performed on the column of ZORBAX Eclipse XDB-C18 with mobile phase of methanol-water at a flow rate of 1.0 ml/min(46∶54,V/V),detection wavelength was 248 nm,column temperature was 30℃,and the injection volume was 10 μl. Compare the contents of ecdysterone in different parts of R. serratulae. RESULTS:The linear range of ecdysterone was 0.12-1.21 μg;RSDs of precision,stability and reproducibility tests were lower than 1.5%;recovery was 98.4%-101.9%(RSD=1.64,n=6). The content of ecdysterone in roots was found at the highest level,followed by tubers,and lower in stems and leaves, and it was not detected in seeds. CONCLUSIONS:The method is simple and rapid with good accuracy and reproducibility,and suitable for the content determination of ecdysterone in different parts of R. serratulae. It is feasible to develop the medicinal parts of R. serratulae from roots to roots,flowers,tubers and buds on tubers.