Box-Behnken设计-响应面法优化桂枝茯苓胶囊的闪式提取工艺

摘要

OBJECTIVE:To optimize the flash extraction technology for Guizhi fuling capsule. METHODS:Using blade speed,liquid-solid ratio,extraction time as investigation factors,using the comprehensive evaluation values consisting of transfer rates of gallic acid,paeoniflorin,benzoic acid,cinnamic acid,benzoyl paeoniflorin,amygdalin as investigation index,single fac-tor test was combined with Box-Behnken design-response surface method to optimize the flash extraction technology for Guizhi ful-ing capsule. Verification test was conducted and compared with conventional decoction extraction method(extracting twice,totally 240 min). RESULTS:The optimal flash extraction technology for Guizhi fuling capsule was using water as extraction solvent with blade speed of 5600 r/min and liquid-solid ratio of 10.5:1,extracting for 60 s. The average comprehensive evaluation value of veri-fication test was 85.40%(n=3),with relative error of 0.15% to the predicted value(85.55%),higher than decoction extraction method(72.65%). CONCLUSIONS:Optimized flash extraction technology for Guizhi fuling capsule is efficient and rapid.%目的:优化桂枝茯苓胶囊的闪式提取工艺.方法:以内刃转速、液固比、提取时间等为考察因素,以没食子酸、芍药苷、苯甲酸、肉桂酸、苯甲酰芍药苷、苦杏仁苷转移率的综合评价值为考察指标,在单因素试验基础上结合Box-Behnken设计-响应面法对桂枝茯苓胶囊闪式提取工艺进行优化;进行验证试验,并与常规的煎煮提取法(提取2次,共用时240 min)进行效果比较.结果:闪式提取桂枝茯苓胶囊的最优工艺为以水为提取溶剂,内刃转速5600 r/min,液固比10.5:1,提取时间60 s.在此条件下验证试验的平均综合评价值为85.40%(n=3),与预测值85.55%的相对误差为0.15%,并高于煎煮提取法(72.65%).结论:优化的闪式提取工艺用于桂枝茯苓胶囊的提取,具有高效、快速的特点.