壮药竹节蓼的质量标准研究

摘要

目的:建立壮药竹节蓼的质量标准.方法:采用薄层色谱法(TLC)对药材样品进行定性鉴别;采用高效液相色谱法对药材样品中槲皮苷进行含量测定:色谱柱为Agilent Zorbax SB-C18,流动相为乙腈-0.1%冰醋酸溶液(20:80,V/V),流速为1.0 mL/min,检测波长为360 nm,柱温为30℃,进样量为10μL;参照2015年版《中国药典》测定药材样品水分、灰分和浸出物的含量.结果:竹节蓼药材的TLC图斑点清晰,分离度好.槲皮苷检测质量浓度线性范围为0.023~0.46 mg/mL(r=0.9991);精密度、稳定性、重复性试验的RSD<2.0%;加样回收率为98.01%~103.05%(RSD=1.71%,n=6).药材样品水分、总灰分、酸不溶性灰分、浸出物和槲皮苷含量分别为6.39%~9.78%、2.82%~8.07%、0.02%~0.27%、14.14%~28.45%、0.09%~0.50%.结论:该研究所建标准可用于壮药竹节蓼的质量控制.%OBJECTIVE:To establish quality standard for Zhuang medicine Homalocladium platycladum. METHODS:TLC method was used for qualitative identification. HPLC method was used to determine the content of quercetrin in samples:Agilent Zorbax SB-C18 column,mobile phase consisted of acetonitrile-0.1% acetic acid(20:80,V/V),flow rate of 1.0 mL/min,detection wavelength of 360 nm,column temperature of 30 ℃,injection volume of 10 μL. The contents of moisture,total ash and extract in samples were determined. referring to Chinese Pharmacopiea(2015 edition). RESULTS:TLC spots of H. platycladum were clear and well-separated without interference from negative control. The linear range of quercetin were 0.023-0.46 mg/mL(r=0.9991). RSDs of precision,stability and repeatability tests were all lower than 2.0%. The average recoveries were 98.01%-103.05%(RSD=1.71%,n=6). The contents of moisture,total ash,extract and quercetrin in samples were 6.39%-9.78%,2.82%-8.07%, 0.02%-0.27%,14.14%-28.45%,0.09%-0.50% respectively. CONCLUSIONS:Established trial can be used for quality control of Zhuang medicine H. platycladum.