漆大姑水提物外用对慢性皮炎-湿疹模型小鼠的改善作用研究

摘要

目的:考察漆大姑水提物外用对慢性皮炎-湿疹模型小鼠的改善作用,为开发一种皮肤科新药提供参考.方法:将60只小鼠按体质量和性别随机分为空白组(蒸馏水)、模型组(蒸馏水)、复方醋酸曲安奈德组(阳性对照,原液)和漆大姑水提物高、中、低浓度组(以生药量计分别为2.0、1.0、0.5 g/mL),每组10只.除空白组外,其余各组小鼠均以2,4-二硝基氯苯诱导复制慢性皮炎-湿疹模型.于实验第3天开始,每天7:00和15:00分别在小鼠右耳正反两面涂抹相应药物2次,每次15μL,连续用药12 d.于实验第10、13、16天,计算小鼠右耳厚度差(与实验前的差值),并记录小鼠30 min内对耳部的搔抓次数;于实验第16天,测定小鼠的耳肿胀度,计算小鼠的胸腺指数和脾指数,并在光学显微镜下观察小鼠耳组织病理学变化.结果:与空白组比较,模型组小鼠右耳厚度差、耳肿胀度、脾指数、搔抓次数均明显增加(P<0.05或P<0.01);耳组织发生表皮棘层明显增厚,细胞明显增生,表皮中可见海绵样水肿等病理学改变.与模型组比较,除漆大姑水提物低剂量组小鼠在实验第16天的右耳厚度差和在实验第10、16天对耳部的抓搔次数以及漆大姑枝叶水提物中、低浓度小鼠胸腺指数减少不显著外,其余组各指标均显著减少(P<0.05或P<0.01);各给药组小鼠耳组织的病理学变化均得到一定改善.结论:漆大姑枝叶水提物外用对小鼠慢性皮炎-湿疹有较好的改善作用,值得进一步开发研究.%OBJECTIVE:To investigate improvement effect of external administration of water extract from Eriocarpous Glochidion on chronic dermatitis-eczema model mice, and to provide reference for developing new dermatological drug. METHODS:A total of 60 mice were randomly divided into blank group (distilled water), model group (distilled water), compound triamcinolone acetonide acetate group (positive control group, original liquid) , high-concentration, medium-concentration and low-concentration groups of water extract from Eriocarpous Glochidion(2.0,1.0,0.5 g/mL,calculated by crude drug),10 mice in each group. Except for blank group,other groups were given 2,4-dinitrochlorobenzene to induce chronic dermatitis-eczema model. Since the third day of the experiment,two sides of right ears of the mice were given relevant medicine twice at 7:00 and 15:00,15 μL each time,for consecutive 12 d. In the tenth,thirteenth,sixteenth day of the experiment,the difference of thickness of right ears of the mice was calculated(the difference value before experiment),the times of scratching in ear of mice were recorded within 30 min. In sixteenth day of the experiment,ear swelling degree of mice was determined,and thymus index and spleen index in mice were calculated. Optical microscope was used to observe the pathological changes of ear tissues of mice. RESULTS:Compared with blank group,the difference of thickness of right ears,ear swelling degree,spleen index and scratching times were increased significantly in model group(P<0.05 or P<0.01). The epidermis of the ear tissue was thickened,the cell proliferation was obvious,and cavernous edema was found in the epidermis. Compared with model group,other indexes in were decreased significantly,except that the difference value of thickness of right ears in sixteenth day of the experiment,scratching times in ninth and fifteenth day of the experiment were decreased slightly in low-concentration group of water extract from Eriocarpous Glochidion and the thymus index and spleen index in medium-concentration, low-concentration groups of water extract from Eriocarpous Glochidion (P<0.05 or P<0.01). The pathological changes of ear tissue were improved in administration groups to certain extent. CONCLUSIONS:External administration of water extract of Eriocarpous Glochidion has good improvement effect against chronic dermatitis-eczema in mice, which is worthy of further development and investigation.