OBJECTIVE: To optimize water extraction technology of Feng-Moutan Cortex. METHODS: L9 (34) orthogonal design was used to optimize soaking time, solid-liquid ratio, extraction time and extraction times in water extraction technology of Feng-Moutan Cortex using comprehensive scores for the peak area percentage and extract yield of 8 ingredients in Feng-Moutan Cortex as gallic acid, paeoniflorin, catechin, paeoniflorin, benzoic acid, benzoyloxypaeoniflorin, benzoylpaeoniflorin and paeonol after normalization as indexes. Verification test was conducted. RESULTS: The optimal water extraction technology was that 20-fold water, soaking for 1 h, extracting twice, 2 h each time. Results of verification test showed that comprehensive scores of 3 times of tests were 65. 98, 68. 85 and 69. 25, respectively. RSDs of each index were lower than 5％ (n=3). CONCLUSIONS: Established comprehensive scoring method can reflect the relative contents of effective components in Feng-Moutan Cortex comprehen- sively, so that optimized extraction technology is reasonable and feasible.%目的:优化凤丹皮水提工艺.方法:采用L9(34)正交试验设计,以凤丹皮中8个成分没食子酸、氧化芍药苷、儿茶素、芍药苷、苯甲酸、苯甲酰氧化芍药苷、苯甲酰芍药苷、丹皮酚的峰面积百分比和浸膏率经归一化法后的综合评分为考察指标,对凤丹皮水提工艺的药材浸泡时间、料液比、提取时间、提取次数进行优化,并进行验证试验.结果:最优水提工艺为加20倍水,浸泡药材1 h, 提取2次、每次2 h.验证试验结果中,3次试验的综合评分分别为65. 98、68. 85、69. 25,各指标的RSD均小于5％(n=3).结论:建立的综合评分方法能较全面地反映凤丹皮的相对有效成分含量,以此优化得到的提取工艺合理、可行.
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