松茸不同提取部位的体外抗肿瘤作用

摘要

Objective:To investigate the anti-tumor effect of different extracts of Tricholoma matsutake Sing. in vitro. Methods:MTT method was employed to study the inhibitory effect of ethanol extracts from different parts of Tricholoma matsutake Sing. and the polysaccharides extracts on HepG-2 cells, MCF-7 cells and Hela cells. FCM assay was used to detect HepG-2 and Hela cell apoptosis induced by the polysaccharides extracts. Results:The polysaccharides extracts of Tricholoma matsutake Sing. could inhibit the prolif-eration of the three tumor cells with IC50 of 53. 77 μg·mL-1(HepG-2), 40. 04 μg·mL-1(Hela) and 100. 65 μg·mL-1(MCF-7), respectively. The inhibitory effect of the polysaccharides extracts showed a time-and dose-dependent manner. The tumor cell apoptosis could be induced by the polysaccharides extracts. Conclusion:The maln anti-tumor effective extracts of Tricholoma matsutake Sing. in vitro is the polysaccharides extracts, which show significant anti-tumor activities in vitro and can induce tumor cell apoptosis.%目的：：研究松茸不同提取部位的体外抗肿瘤活性。方法：采用MTT法测定松茸乙醇提取物各部位及多糖部位对HepG-2细胞、Hela细胞和MCF-7细胞的抑制作用；采用流式细胞术检测多糖部位诱导HepG-2细胞和Hela细胞凋亡的作用。结果：松茸多糖部位体外对3种肿瘤细胞的IC50分别为53.77μg·ml-1(HepG-2)、40.04μg·ml-1(Hela)、100.65μg·ml-1(MCF-7)，抑制作用均优于其他各部位，且对HepG-2细胞和Hela细胞抑制作用呈现良好的时间与浓度依赖性；松茸多糖部位能诱导HepG-2细胞和Hela细胞凋亡。结论：松茸多糖部位能显著抑制HepG-2和Hela等肿瘤细胞的生长，具有诱导凋亡的作用，为松茸抗肿瘤的主要活性部位。