固相萃取结合高效液相色谱法测定人血浆中酒石酸唑吡坦的浓度

摘要

Objective:To establish a method for the determination of zolpidemtartrate in human plasma by SPE-HPLC. Methods:The samples were extracted by solid phase with a Hypersil BDS C18 column and determined by an internal standard method. The mobile phase was methanol:acetonitrile:water (45 ∶45 ∶110), the flow rate was 1. 0 ml·min-1, the column temperature was 35℃, and the detection wavelength was 254nm. Results:The linear relationship of zolpidemtartrate was within the range of 0. 511 5-4. 092 0 μg· ml-1 . The relative recovery was greater than 90%. The intra-and inter-day RSDs were all less than 10%. Conclusion:The method is quite simple, quick and accurate with little interference, which is suitable for the clinical determination of zolpidemtartrate.%目的：：建立固相萃取结合高效液相色谱法测定人血浆中酒石酸唑吡坦浓度。方法：血样经固相萃取法提取,采用大连依利特色谱柱(Hypersil BDS C18),流动相：甲醇-乙腈-水(45∶45∶110),流速1.0ml·min-1,柱温：35℃；检测波长：254 nm,内标法定量。结果：血浆中酒石酸唑吡坦浓度在0.5115~4.0920μg·ml-1线性关系良好,相对回收率大于90%,精密度日间、日内RSD均小于10%。结论：该测定方法简单、快速、准确、干扰小,适用于临床测定酒石酸唑吡坦药物浓度。