利伐沙班对下肢动脉硬化闭塞症大鼠血管内皮功能及p38MAPK/NF-κB通路的影响

摘要

目的:探究利伐沙班对下肢动脉硬化闭塞症(ASO)大鼠血管内皮功能及p38丝裂原活化蛋白激酶(p38MAPK)/核因子κB(NF-κB)通路的影响.方法:将大鼠分为假手术组、模型组、利伐沙班低剂量组、利伐沙班中剂量组、利伐沙班高剂量组,给药2周后检测各组大鼠血清中一氧化氮(NO)和内皮素(ET-1)含量;检测血清中内皮细胞的数量;检测动脉血管中p38MAPK、p-p38MAPK、NF-κB表达情况.结果:与模型组相比,利伐沙班组静脉血中内皮细胞数量减少;利伐沙班组血清中NO显著高于模型组,ET-1显著低于模型组(P<0.05);利伐沙班组动脉中p-p38MAPK的表达量显著低于模型组,NF-κB的表达量显著高于模型组(P <0.05),呈剂量依赖性.结论:利伐沙班能够改善 ASO 大鼠血管内皮功能,推测是通过调控p38MAPK / NF-κB通路实现的.%Objective:To explore the influence of rivaroxaban on vascular endothelial function and p38MAPK/NF-κB pathway in the rats with arteriosclerosis obliterans. Methods:The rats were divided into the sham operation group,the model group,rivaroxaban low dose group,rivaroxaban medium dose group and rivaroxaban high dose group. The contents of nitric oxide (NO) and endothelin (ET-1) in serum were detected, the number of endothelial cells in serum was studied, and the expression level of p38MAPK, p-p38MAPK and NF-κB were measured. Results:Compared with that of the model group, the number of endothelial cells in venous blood of rivaroxaban groups decreased. The content of NO in serum of rivaroxaban groups was significantly higher than that of the model group,and the content of ET-1 was significantly lower than that of the model group(P<0.05). The expression level of p-p38MAPK in arterial vessels of rivaroxaban groups was significantly lower than that of the model group,and the expression level of NF-κB was sig-nificantly higher than that of the model group(P<0.05),and the effects were all in a dose-dependent manner. Conclusion:Rivarox-aban can improve vascular endothelial function in the rats with arteriosclerosis obliterans,and the effects may be achieved by modula-ting the p38MAPK / NF-κB pathway.