高效液相色谱法测定丹墨胶囊中丹酚酸B含量

摘要

目的 建立测定色谱丹墨胶囊中丹酚酸B含量的高效液相色谱法.方法 色谱柱为Ecosil C18柱(200 mm×4.6 mm,5 μm),流动相为乙腈-甲醇-甲酸-水(10:30:1:59),检测波长286 nm.结果 丹酚酸B进样量在0.120 042～2.400 84 μg范围内与峰面积呈良好的线性关系,平均回收率为103.40%,RSD为0.70%(n=6).结论 该方法简便、灵敏、准确,可用于丹墨胶囊的质量控制.%Objective To eslablish a melhod for ihe conlenl delerminalion of salvianolic acid B in Danmo Capsules by HPLC. MethodsColumn was Ecosil CihV 200 mm x4. 6 mm, 5 |Jjti r, mobile phase was acelonilnle - melhanol - formic acid - waler 10 ■ 30 ■ 1 ■ 59 /, UV deleclion wavelenglh was al 286 nm. Results There was a good linear relalionship of salvianolic acid B in ihe range of 0. 120 042 - 2. 400 84 |_Lg. The average recovery rale was 103. 40% , RSD was 0. 70% (n = 6 ). CODClusiOD This melhod is simple, accurale and reliable. 11 can be used for qualily conlrol of Danmo Capsules.