目的 观察X染色体连锁的凋亡抑制蛋白(XIAP蛋白)对米非司酮诱导宫颈癌HeLa细胞凋亡的影响.方法 XIAF重组质粒转染HeLa细胞,共聚焦显微镜观察其在细胞内的分布;流式细胞仪检测米非司酮处理和未处理的各组细胞凋亡率.结果 XIAP在HeLa细胞中弥漫分布于细胞核和细胞浆,米非司酮可诱导HeLA细胞凋亡,XIAP能抑制由米非司酮诱导的HeLa细胞凋亡.结论 米非司酮能促进HeLa细胞凋亡,XIAP能抑制由米非司酮诱导的HeLa细胞凋亡.%Objective To investigate the effects of mifepristone -induced apoptosis in uterine cervix cancer cell line HeLa and the influence of mifepristone on the effectiveness of XIAP in uterine cervix cancer cells. Methods Human uterine cervix cancer cells lines HeLa were used for the current project. Expression,intracellular localization and function of the XIAP in HeLa cells were observed by Laser scanning confocal microscope after transfection. HeLa cells were induced by mifepristone. At the same time,the apoptosis were analyzed for different groups by FCM. Results Expression of DsRed2 - XIAP fusion proteins in HeLa cells were observed in both nucleus and cytoplasm of HeLa cells. HeLa cells which were induced by mifepristone showed partly apoptosis. The DsRed2 - XIAP fusion protein can inhibit apoptosis of HeLa cells by inducing of mifepristone. Conclusion The mifepristone can promote apoptosis of HeLa cells. The DsRed2 - XIAP fusion protein can inhibit apoptosis of HeLa cells by inducing of mifepristone.
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