大孔树脂精制杭白菊总黄酮的上样条件研究

摘要

目的 优选D-101大孔树脂精制杭白菊总黄酮工艺中的最佳上样条件.方法 将影响树脂吸附性能的3个因素上样量、流速和pH设计成3因素3水平试验,采用L9(34)表正交试验,以树脂吸附总黄酮含量、乙醇洗脱总黄酮含量和固体总黄酮含量为评价指标,选择最佳条件.结果 确定最佳上样条件为上样量20 mL、pH为5、流速2 BV/h.结论 该最佳上样条件可应用于大孔树脂精制杭白菊总黄酮的上样条件研究.%Objective To optimize D — 101 macroporous resin for obtaining the best sampling conditions to refine chrysanthemum total flavonoids. Methods 3 factors influencing resin adsorption ability,the sample volume,flow rate and sample solution pH value,were designed to the 3 factors 3 levels test. Adopting the L9(34) table orthogonal experiment,the total flavonoid content by resin adsorption, total flavonoids content by ethanol eluting and solid total flavonoid content were used as the evaluation indexes for screening the best conditions. Results The best optimum sampling conditions were A3B1G3,I. E. ,the sample volume of 20 mL,pH 5 and the velocity of 2 BV/h. Conclusion The best sampling conditions can be used in the sampling condition study for refining chrysanthemum total flavonoids by macroporous resin.