Objective To evaluate the medical staff about the relative knowledge of rational use of antibacterial drug in our hospital, and to further improve the level of antibacterial drugs standardized management in our hospital, to ensure safe drug use of patients. Methods The questionnaire was designed according to the national and military related laws and regulations and the basic knowledge of antimi-crobial drugs, it was conducted among doctors、nurses and pharmacists (including trainee) in our hospital, the result of survey was ana-lyzed statistically. Results A total of 305 questionnaires were issued, and 297 questionnaires were recovered including 7 invalid ques-tionnaires, the effective recovery rate is 95. 08% . On the whole, the highest rate of cognition of rational use of antimicrobial drugs is pharmacists, followed by doctors, and nurses. There is a big difference in cognition about the specific knowledge: there are 65. 63% of doctors, 40. 37% of nurses and 85. 71% of pharmacists were clear about the concept of antimicrobial drugs; only 61. 25% of doctors, 50. 46% of nurses and 100. 00% of pharmacists are aware of the hierarchical management of antimicrobial drugs; 60. 63% of doctors, 44. 04% of nurses and 90. 48% of pharmacists are aware of the administration time about preventive medication for type I incision op-eration; lecture was the main demand approach for the knowledge of antibacterial drugs, which was accounted for 86. 90% of the medical personnel. Conclusion Our hospital medical staff awareness of the rational use of antimicrobial drugs is low. Suggestions that the an-timicrobial management team consists of medical department, infection control department, inspection department and medical departments and other personnel was established. It is suggested to enhance the training of antimicrobial drug related knowledge for the medical staff, improve their cognition and alertness of antimicrobial agents so as to reduce the drug resistant bacteria and the occurrence of ADR, improve the level of rational use of antimicrobial agents our hospital.%目的：评估医院医务人员对抗菌药物合理使用相关知识的掌握情况，为提高医院抗菌药物规范化管理水平、保障患者用药安全提供依据和参考。方法设计调查问卷，对医生、护士、药师进行认知调查，就结果进行统计、分析。结果共发放问卷305份，回收问卷297份，其中无效问卷7份，有效回收率为95.08%。药师对抗菌药物合理使用相关知识认知率最高，其次是医生和护士；具体专业知识的认知情况差异大，有65.63%的医师、40.37%的护士和85.71%的药师明确抗菌药物的概念；对我院抗菌药物分级管理知晓的医生仅为61.25%，护士为50.46%，药师为100.00%；对Ⅰ类切口手术预防用药的给药时间知晓的医生为60.63%，护士为44.04%，药师为90.48%；86.90%的医务人员对抗菌药物知识的需求途径主要是专题讲座。结论医院医务人员对抗菌药物的合理使用认知度较低。建议组成抗菌药物管理小组，加强对医务人员抗菌药物相关知识的培训，提高其抗菌药物的认知程度和警惕性，减少耐药菌及其不良反应的发生，进而提高医院抗菌药物合理使用水平。
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