披针骨牌蕨总黄酮对高糖诱导血管内皮细胞氧化损伤的保护作用

摘要

目的 探讨披针骨牌蕨总黄酮对血管内皮细胞氧化损伤的保护作用.方法 建立高糖诱导的人脐静脉血管内皮细胞(HUVEC)氧化损伤模型进行药物试验,分为正常对照组(葡萄糖质量浓度为5.5 mmol/L)、高糖损伤模型组(葡萄糖质量浓度为40 mmol/L)、提取物组(披针骨牌蕨总黄酮质量浓度分别为100,200,400 mg/L)、阳性对照组(维生素C质量浓度为30 mg/L).以四甲基偶氮唑蓝(MTT)比色法测定细胞存活率,以试剂盒测定细胞中乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、一氧化氮合酶(NOS)释放量及活性的变化.结果 提取物组MDA释放量明显降低,LDH释放量及活性下降,SOD的活性明显增高,NO和NOS生成量及活性明显增高,具有显著性差异(P<0.05),且在一定质量浓度范围内与披针骨牌蕨总黄酮呈剂量依赖性关系.结论 披针骨牌蕨总黄酮对高糖诱导HUVEC氧化损伤具有一定的保护作用,其作用机制可能与机体抗氧化酶活性增加、自由基清除、抗氧化能力提高有关.%Objective To investigate the protective effect of total flavonoids from Lepiologrammitis Diversa on high glucose-induced ox-idative damage in vascular endothelial cells. Methods To establish oxidative damage models of human umbilical vein endothelial cells ( HUVEC ) induced by high glucose and to carry out drug test. The models were divided into the normal control group ( the mass con-centration of glucose was 5. 5 mmol/L ) , high glucose injury group ( the mass concentration of glucose was 40 mmol/L ) , extract group ( the mass concentration of total flavonoids from Lepiologrammitis Diversa were 100, 200, 400 mg/L ) and positive control group ( the mass concentration of vitamin C was 30 mg/L ) . The cell survival rate was measured by MTT assay, the changes of release and activity of LDH, MDA, SOD, NO and NOS in cells were determined by diagnostic reagent kit. Results In the extract group, the release amount of MDA was decreased significantly, the release amount and activity of LDH was decreased significantly, the activity of SOD was in-creased significantly, and the quantity and activity of NO and NOS were increased significantly ( P < 0. 05 ) , in a certain concentration range, and the total flavonoids from Lepiologrammitis Diversa showed dose dependent relationship in a certain mass concentration range. Conclusion Total flavonoids from Lepiologrammitis Diversa have protective effect on oxidative damage induced by high glucose in HUVEC. The preliminary mechanism may be related to the increase of antioxidase activity, the scavenging of free radicals and the in-crease of antioxidant capacity.