清补通络浓缩丸提取工艺优化研究

摘要

目的 优选清补通络浓缩丸提取工艺.方法 采用高效液相色谱(HPLC)法测定黄芩苷含量,以黄芩苷含量及出膏率为考察指标,正交试验优选最佳提取工艺.结果 最佳提取工艺为料液比1:10,提取2次,每次1 h;在此条件下,黄芩苷含量为4.49 mg/g,出膏率为4.84％.结论 优选的清补通络浓缩丸提取工艺合理、稳定、效果良好,可为清补通络浓缩丸及其他中药新药的生产提供试验依据.%Objective To optimize the extraction process of Qingbutongluo Concentrated Pills. Methods The content of baicalin was determined by HPLC,and the optimum extraction process was optimized by orthogonal test with the content of baicalin and the yield of extraction as the indexes. Results The optimum extraction process was as follows:the ratio of material to liquid was 1 :10 with 2 times of extraction,1 h each time. Under this condition,the content of baicalin was 4. 49 mg/g,and the yield of extraction was 4. 84％. Conclusion The optimized extraction process is reasonable,stable and effective,which can provide the experimental basis for the pro-duction of Qingbutongluo Concentrated Pills and other new medicines of traditional Chinese medicine.