血清内源性硫化氢在支气管哮喘患儿中的表达及与呼吸功能的相关性分析

摘要

Objective To explore expressive level of endogenous hydrogen sulfide (H2S) in serum in children with bronchial asthma and its correlativity with respiratory function. Methods 50 children with bronchial asthma in acute at-tack stage from December 2013 to December 2014 in our hospital was selected as the acute attack group,50 children with bronchial asthma in remission stage were selected as the remission group and 50 cases of healthy children were selected as the control group during the same period.Endogenous H2S was detected with sensitive sulphur electrode and respiratory function was detected with lung function diagnostic system in every groups.The expression level of endoge-nous H2S,the index of forced vital capacity(FVC),forced expiratory volume in 1 second (FEV1%),maximal mid-expiratory flow (MMEF) among three groups was compared respectiveiy,and relativity of H2S with FVC,FEV1% and MMEF was an-alyzed in acute attack group. Results The level of H2S in the acute attack group and the remission group was lower than that in the control groups,with significant difference (P<0.05).The level of H2S in the acute attack group was lower than that in the remission group,with significant difference (P<0.05).The level of FVC,FEV1% and MMEF in the acute attack group and the remission group was lower than that in the control groups,with significant difference (P<0.05).The level of FVC,FEV1% and MMEF in the acute attack group was lower than that in the remission group,with significant difference (P<0.05).The level of H2S in serum was positive correlation with FVC,FEV1% and MMEF in acute attack group. Conclusion Expressive level of endogenous hydrogen sulfide in children with bronchial asthma is decreased,and which has correlative with respiratory function,detection of endogenous hydrogen sulfide has certain clinical signifi-cance for predicting lung function.%目的：探讨气体信号分子内源性硫化氢在支气管哮喘患儿血清中的表达情况及其与呼吸功能的相关性。方法选取2013年12月~2014年12月本院收治的50例支气管哮喘急性发作期患儿作为急性发作组,50例支气管哮喘缓解期患儿作为缓解组,选取同期的50例健康小儿作为对照组,采用敏感硫电极法检测血清硫化氢表达水平,采用肺功能检测仪检测各组呼吸功能,比较3组的内源性硫化氢表达水平及用力肺活量(FVC)、第一秒最大呼气率(FEV1%)、最大呼气中期流速(MMEF)等指标,分析急性发作期患儿FVC、FEV1%、MMEF与内源性硫化氢表达水平的相关性。结果急性发作组和缓解组的血清内源性硫化氢水平显著低于对照组,急性发作组的血清内源性硫化氢水平显著低于缓解组,差异有统计学意义(P<0.05)。急性发作组和缓解组的FVC、FEV1豫及MMEF水平显著低于对照组,急性发作组的FVC、FEV1豫及MMEF水平低于缓解组,差异具有统计学意义(P<0.05)。急性发作组内源性硫化氢表达水平同用力肺活量、第一秒最大呼气率、最大呼气中期流速呈现正相关。结论内源性硫化氢在支气管哮喘患儿中的表达水平降低,并且和呼吸功能相关,检测内源性硫化氢水平对于预测肺功能具有一定的临床意义。