XE-5000全自动血细胞分析仪检测白细胞分类与人工镜检结果对比分析

摘要

目的：探讨XE-5000全自动血细胞分析仪检测白细胞（WBC）分类的准确性。方法随机收集本院2012年6月~2013年6月门诊、住院患者及健康体检者共208例，根据年龄进行分组，其中成人组100例、儿童组60例、新生儿组48例，分别用Sysmex XE-5000与人工镜检法对不同年龄组的静脉血进行WBC分类，采用统计学软件对两种方法的结果进行对比分析，并进行相关性分析。结果除嗜碱粒细胞外，成人组仪器法与人工镜检法检测WBC分类计数结果比较，差异无统计学意义（P>0.05），且呈正相关（P<0.05）。除单核细胞、嗜碱粒细胞外，儿童组及新生儿组WBC分类计数结果比较，差异无统计学意义（P>0.05），且呈正相关（P<0.05）。结论 Sysmex XE-5000全自动血细胞分析仪测定WBC分类具有良好的性能，适用于对大批量标本进行快速有效的检测，但不能完全取代人工镜检法，两者结合，才能保证检验结果的准确性和可靠性。%Objective To study the accuracy of XE-5000 automatic hematology analyzer in detecting the white blood cell (WBC) classification. Methods 208 cases of outpatients,inpatients and subjects of health examination treated in our hospital from June 2012 to June 2013 were randomly selected and divided into the adult group (100 cases),the children group (60 cases) and the neonate group (48 cases) according to age.The WBC in venous blood of the patients in differ-ent groups were classified respectively by Sysmex XE-5000 method and artificial microscopy method.The results of the two methods were compared and analyzed by using statistical software,and the correlation analysis was carried out. Re-sults In addition to the basophils,the difference of the count results of the WBC classification in the adult group detect-ed by using instrument method and artificial microscopy method was not significant (P>0.05),and had a positive correla-tion (P<0.05).In addition to the monocytes and basophils,the difference of the count results of the WBC classification in the children group and neonate group was not significant (P>0.05),and had a positive correlation (P<0.05). Conclusion Using the Sysmex XE-5000 automatic blood cell analyzer to detect the white blood cell classification has a good per-formance and it is suitable to detect a large quantity of samples quickly and effectively,but it can not completely re-place the artificial microscopy.If the two methods are combined,it can ensure the accuracy and reliability of test results.