银杏叶提取物对慢性低O2高CO2大鼠空间学习记忆的影响

摘要

目的：探讨银杏叶提取物对慢性低O2高CO2大鼠空间学习记忆的变化及影响。方法建立慢性低O2高CO2大鼠肺动脉高压模型。将SD大鼠72只，随机分为正常对照组（NC组）、慢性低O2高CO24周组（4HH组）、慢性低O2高CO24周组垣EGb高剂量（100 mg/kg）组[4HH+EGb（H）组]、慢性低O2高CO24周组垣EGb低剂量（50 mg/kg）组[4HH+EGb（L）组]；大鼠空间学习记忆检测，采用经典Morris水迷宫结合跳台作业。结果4HH组大鼠学习与记忆能力下降明显，表现为游泳逃避潜伏期及游泳总距离延长，跳台作业错误次数明显增加；银杏叶提取物不同剂量干预组[4HH+EGb（H）组、4HH+EGb（L）组]逃避潜伏期及游泳总距离缩短，跳台错误次数减少。结论银杏叶提取物对慢性低O2高CO2大鼠空间学习记忆下降有改善或提高作用。%Objective To observe changes of spatial learning-memory in rats with chronic hypoxic hypercapnia and the effect of gingkgo biloba extra. Methods After established the rat model of chronic hypoxic hypercapnia,seventy-two rats were randomly divided into four groups normal control (NC),hypoxic-hypercapnia 4-week (4HH),hypoxic-hy-percapnia 4-week+gingkgo biloba extra (EGb)high dose(100 mg/kg)group[4HH+EGb(H)] and hypoxic-hypercapnia 4-week+EGb low dose (50 mg/kg) group[4HH+EGb(L)]. Praxiology in rats was asessed by the Morris water maze and step down test. Results The spatial learning-memory in rats exposed to chronic hypoxic-hypercapnia 4-week(4HH group)were displayed significant impairment in their performance,the longer mean escape latencies and swim path dis tances,the more error times. 4HH+EGb(H) and 4HH+EGb(L)groups shortened the reaction time of leaning, pro longed the latent time of memory, reduced times of mistakes. Conclusions EGb can enhance the capacity of learning-memory in the rats exposed chronic hypoxic hypercapnia.