miR-196a在下咽鳞状细胞癌血浆中的表达及其临床意义

摘要

Objective To investigate the relationship between the expression level of miR-196a in plasma and the clin-icopathologic factors of HSCC. Methods Paired plasma specimens from HSCC patients and 50 healthy people were collected. Plasma total RNA was extracted from 57 HSCC patients and 50 healthy people ,and the expression level of miR-196a was measured by quantitative real time PCR. The clinicopathological features between the plasma expression level of miR-196a were analyzed. ROC curve were constructed to evaluate the diagnostic value. Results miR-196a exhi-biting higher expression in pre-operation HSCC plasma with △Ct=9.11(5.69,12.43)compared with post-operation HSCC plasma with△Ct=12.69(9.16,14.84)and healthy group with△Ct=12.85(11.18,14.91)(P<0.01). There were no significant differences between the expression level of miR-196a in healthy group and post-operation group (P>0.05). miR-196a levels in plasma were correlated with grade of differentiation (P<0.05), lymph node metastasis(P<0.01), and clinical stage(P<0.05), while there was no relationship with age and smoking history(P>0.05). The areas under the ROC curves were 0.755. The cut-off point of△Ct was 10.84,with the 95%CI=0.66-8.85(P<0.01). Conclusion Plasma expression level of miR-196a have realationship with clinical stage ,lymph node matastasis and grade of differentiation. miR-196a may have involved in carcinogenesis and tumor development of HSCC. Plasma miR-196a could be potential minimally invasive biomarkers for HSCC patients.%目的：探讨miR-196a在下咽鳞状细胞癌（HSCC）患者血浆中的表达水平及其与下咽癌临床因素的关系，明确miR-196a 的诊断价值及临床意义。方法利用57例临床确诊HSCC患者血浆配对样本，健康体检者血浆样本50例，从血浆标本中提取总RNA，应用实时荧光定量PCR（quantitative real time PCR，qRT-PCR）方法检测miR-196a在血浆中的表达水平，收集57例HSCC患者病理、临床资料，统计分析血浆miR-196a水平和临床病理特征的关系。构建受试者工作特征曲线（receiver operating characteristic curve，ROC），探讨血浆miR-196a的诊断价值。结果在HSCC患者中术前miR-196a的水平9.11（5.69，12.43）显著高于术后12.69（9.16，14.84）及健康体检者12.85（11.18，14.91）（P<0.01）；血浆标本中HSCC患者术后miR-196a水平与健康体检者比较无显著差异（P>0.05）。miR-196a血浆中的表达水平和分化程度（P<0.05）、淋巴结转移（P<0.01）、临床分期（P<0.05）有显著相关性；与年龄、吸烟史无关（P>0.05）。 ROC曲线下面积为0.755，截断（cut-off）值为△Ct=10.84，95%CI：0.66～8.85（P<0.01）。结论血浆miR-196a血浆表达水平与HSCC患者的肿瘤分期、淋巴结转移、病理类型等若干临床特征相关。miR-196a有望作为HSCC微创诊断分子标志物。