机械性创伤致继发性肺损伤大鼠肺组织中叉头盒转录因子A1表达的变化及意义

摘要

Objective To study the effect of secondary lung injury caused by mechanical trauma on the expression change of Forkhead box protein Al ( FoxAl) at both mRNA and protein levels. Methods Forty male Wistar rats were randomly divided into sham group and trauma group, each group 20, Noble-Collip drum was used to establish mechanical trauma rat model. Trie total RNA and protein of lung tissue was extracted after modeling of abdominal aortic blood sampling time points were killed. The expression of FoxAl mRNA in rats' lung tissue was observed by real-time RT-PCR, the expression of FoxAl protein was detected by Western Blot. Results The expression of FoxAl protein in trauma group was higher than that in sham group( sham group 0.59 ± 0.04, trauma group 0.91 ± 0.03, P < 0.01). Conclusion FoxAl plays an important role in secondary lung injury caused by mechanical trauma.%目的 研究机械性创伤致继发性肺损伤大鼠肺组织中叉头盒转录因子(Fox)A1表达的变化及意义.方法 将雄性Wistar大鼠40只完全随机分为伪创伤组和创伤组,每组20只.利用Noble-Collip创伤仪制备机械性创伤模型.全部大鼠均于造模后6h腹主动脉采血处死,提取肺组织的总RNA和总蛋白.逆转录聚合酶链反应检测FoxA1 mBNA的表达,蛋白质印迹检测FoxA1蛋白的表达.结果 创伤组FoxA1mRNA表达水平高于伪创伤组(P<0.01),是伪创伤组的9.25倍;FoxA1蛋白表达:伪创伤组(0.59±0.04),创伤组(0.91±0.03),创伤组FoxA1蛋白表达水平高于伪创伤组,差异有统计学意义(P<0.01).结论 FoxA1在机械性创伤致继发性肺损伤中起着重要的作用.