系统性红斑狼疮患者外周血未成熟单核细胞来源树突状细胞诱导调节性T细胞分化的体外实验研究

摘要

Objective To observe the differentiation of regulatory T cells (Treg) to CD4+ CD25+ forkhead box P3 (FOXP3) + Treg cells induced by immature monocyte-derived dendritic cells (MDDC) in peripheral blood of patients with systemic lupus erythematosus (SLE) in vitro.Methods The immature MDDC in peripheral blood from healthy people(control groups) and SLE patients (SLE groups) were separated and cultured in vitro.The immature MDDC and autologous CD4+ T cells were cocuhured, and the proportion of FOXP3+ CD4+ CD25+ Treg cells were measured by flow cytometry.Results The proportion of FOXP3+ CD4+ CD25+ Treg cells in CD4+ T cells was significantly lower in SLE groups than that in control group [(2.83 ± 0.30) ％ vs (5.38 ± 0.63) ％] (P ＜ 0.05);after being cocultured with immature MDDC, the proportion of FOXP3+ CD4+ CD25+ Treg cells was significantly increased in control group [(8.12 ± 0.70) ％] (P ＜ 0.05), but not in SLE group [(3.28 ± 0.27) ％] (P ＞ 0.05).Conclusion The MDDC in patients with SLE shows a defective capability to induce the differentiation of CD4+ Treg cells to CD4+ CD25+ FOXP3+ Treg cells.%目的 探讨系统性红斑狼疮(SLE)患者外周血未成熟单核细胞来源树突状细胞(MDDC)在体外诱导CD4+调节性T细胞(Treg)向人叉头框蛋白P3(FOXP3)+CD4+ CD25+ Treg细胞分化的能力.方法 抽取SLE患者(SLE组)及健康人群(对照组)的外周血,于体外分离培养未成熟MDDC;将未成熟MDDC与CD4+ T细胞共育,采用流式细胞术检测FOXP3+ CD4+ CD25+ Treg细胞的比例.结果 SLE组外周血CD4+ T细胞中FOXP3+CD4+CD25+ Treg细胞比例明显低于对照组[(2.83±0.30)％比(5.38±0.63)％](P＜0.05);CD4+ T细胞与未成熟MDDC共育后,SLE组FOXP3+ CD4+ CD25+ Treg细胞比例为(3.28±0.27)％,与共育前比较无明显增加(P＞0.05),对照组FOXP3 +CD4+ CD25+ Treg细胞比例为(8.12±0.70)％,较共育前明显增加(P＜0.05),2组间比较,差异有统计学意义(P＜0.05).结论 SLE患者未成熟MDDC诱导自体CD4+ Treg细胞向FOXP3 +CD4+ CD25+ Treg细胞分化能力缺陷.