非毒性结节性甲状腺肿蛋白质表达差异的初步研究

摘要

Objective To analyze the comparison of overall protein expression between nontoxic nodular goiter (NTNG) tissues and normal thyroid tissues, and to explore the difference of protein expression of NTNG. Methods Total proteins of NTNG tissues and normal thyroid tissues were extracted and separated via two-dimensional gel electrophoresis; the proteins were analyzed via MALDI-TOF/TOF mass spectrum, relevant proteins were matched in searching of database and then identified, and finally the information of different proteins were obtained. Results Nine differentially expressed protein spots which were statistically significant were obtained via primary screening and analysis of mass spectrum, four of which showed high expression in NTNG tissues, while five of which showed low expression. Conclusion There are various types of differentially expressed proteins between NTNG and normal thyroid. Extracellular matrix, cytokines, trans-cellular transduction of receptor signals are likely to be involved in the onset and development of NTNG.%目的：比较非毒性结节性甲状腺（NTNG）组织和正常甲状腺组织整体蛋白质表达，以研究非毒性结节性甲状腺组织蛋白质表达差异。方法提取NTNG甲状腺组织和正常甲状腺组织总蛋白，利用双向凝胶电泳技术分离各组总蛋白；经MALDI- TOF / TOF质谱分析，搜索数据库，寻找匹配的相关蛋白质，鉴定蛋白质，获得差异蛋白质的信息。结果经过初步筛选和质谱分析共获得9个具有统计学意义的表达差异蛋白点，其中4个在非毒性结节性甲状腺高表达，5个低表达。结论 NTNG与正常腺体组织间存在多种差异表达的蛋白质，可能通过细胞外基质、细胞因子、受体信号转细胞信号传递等参与NTNG的发生和发展。