氨磷汀对免疫性血小板减少性紫癜动物模型小鼠的干预作用

摘要

Objective To study the effect of amifostine on mice models of idiopathic thrombocytopenic purpura(ITP). Methods Guinea pig anti mice platelet serum(GP-APS) was diluted with saline(1:4),and intravitreal injected into mice once in 2 days for 10 days.Two dosage of amifostine was intravitreal injected into mice in 10 days since mice models of ITP were established. Results Amifostine can enhance the amount of platelets,promote the mature of megakaryocyte of mice significantly and improve the histological pathology of spleen. Conclusion Amifostine is an effective and safety medicine in treating ITP, and can be used widely in clinic.%　　目的探讨氨磷汀对免疫性血小板减少性紫癜（ITP）动物模型小鼠血小板、脾脏系数、骨髓巨噬细胞的影响。方法通过获取BALB/C 小鼠血小板抗体，接种到豚鼠身上，获取豚鼠抗小鼠血小板抗体（APS）。将APS腹腔注射BALB/C 小鼠建立免疫性血小板减少性紫癜动物模型，使用氨磷汀腹腔注射进行治疗，观察氨磷汀对模型小鼠的血小板、脾脏系数、骨髓巨噬细胞的影响。结果氨磷汀给药后均使ITP模型小鼠血小板计数上升、脾脏系数减少、骨髓成熟巨核细胞增多，与模型组比较差异有统计学意义（P＜0.01）。结论氨磷汀对免疫性血小板减少性紫癜有较为明显的治疗效果。