锌对酒精性肝损害小鼠保护作用的研究

摘要

Objective: To study the protective effects of Zn to athonol liver injury in mice. Methods: 50 healthy mice were divided into five groups randomly and 10 mice in each group, normal control group, alcohol control group and low, middle,high dose Zinc Gluconate groups. The mice in the nomal control group and alcohol model control group drank running water in it. Other mice drank solution containing different dose of Zinc Gluconate. Mice were poured in alcohol after 30 days and executed after 16 hours. Biochemical indidicators of mice in each group were measured. Biochemical indicators ineluded AST, ALT, the activity of GSH-Px and content of MDA in rat livers. Results: Compared with normal control group,ALT, AST increased significantly (P＜0.05, P＜0.01). MDA increased significantly and GSH-PX decreased significantly in alcohol control group (P＜0.01). Compared with alcohol control group, ALT of high dose Zinc Gluconate group decreased significantly (P＜0.05). AST of middle and high dose Zinc Cluconate decreased significantly (P＜0.05), all the three groups decreased MDA signnificantly (P＜0.05, P＜0.01). Conclusion: Drinking alcohol can injure enzyme of liver organize. Different dose of Zinc (Gluconate can protect acute alcohol-induced liver damage significantly.%目的:探讨锌对酒精性肝损害小鼠是否具有保护作用.方法:将50只小鼠随机分为5组,每组各10只,分别为空白对照组、白酒对照组和葡萄糖酸锌低、中、高剂量组.其中,空白对照组和白酒对照组饮用自来水;其他各组分别饮用自由溶于水的不同剂量的葡萄糖酸锌溶液.第30天时对小鼠进行空腹白酒灌胃实验,然后处死小鼠,测定肝匀浆中谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)含量及血谷草转氨酶(AST)、谷丙转氨酶(ALT).结果:与空白对照组相比,白酒对照组AST、ALT显著升高(P<0.05或P<0.01),MDA显著性升高、GDH-Px活力显著下降(P<0.01).与白酒对照组相比,高剂量锌组使ALT显著下降(P<0.05),中、高剂量锌组使AST显著下降(P<0.05).各剂量锌组均可使MDA下降,差异有统计学意义(P<0.05或P<0.01).结论:白酒可造成肝组织酶异常,对于急性酒精性肝损害小鼠,葡萄糖酸锌均可对肝脏起到保护作用.