线栓法建立局灶性脑缺血模型的方法与探讨

摘要

Objective To investigate the method and influence factors of establishing focal ischemia model of rat suc-cessfully. Methods Rats were divided into two groups, 250-300 g group and 350-400 g group. In order to observe the influence of fasting on the model, 250-300 g group was subdivided into two groups, control group (n=10) and fasting group (n=10). In order to observe the influence of the bolt line diameter on the model, 350-400 g group was sub-divid-ed into two groups, thin bolt line group (n=10) and bold bolt line group (n=10). The bolt line entered from a cut of the common carotid artery and moved in the intracranial internal carotid artery and at last reached to the starting positions of middle cerebral artery to block blood flow. The nerve function defect score and TTC staining were used to evaluate the model. Results In the 250-300 g group, the success rate was high and mortality of the fasting group (11.1%) was lower than the control group (62.5%) (P<0.05). In the 350-400 g group, mortality was low and the success rate of the bold bolt line group (80%) was higher than the thin bolt line group (20%) (P< 0.05). Conclusion Fasting can reduce animal mortality. Selecting the bolt line according to the weight of rats can increase success rate.%目的：探讨成功建立大鼠局灶性脑缺血模型的有效方法及影响因素。方法将大鼠分为250~300 g和350~400 g两个体重组。为观察禁食对造模的影响，250~300 g体重组又分为对照组(n=10)和禁食组(n=10)；为观察栓线直径对造模的影响，350~400 g体重组又分为细栓线组(n=10)和粗栓线组(n=10)。栓线经颈总动脉、颈内动脉进入大脑中动脉的起始部位阻断大脑中动脉的血流。术后通过神经功能缺损评分和氯化三苯基四氮唑(TTC)染色对该模型进行评价。结果在250~300 g体重组，造模成功率高，禁食组死亡率(11.1%)明显低于对照组(62.5%)(P<0.05)；在350~400 g体重组，死亡率低，粗栓线组造模成功率(80%)明显高于细栓线组(20%)(P<0.05)。结论术前禁食可降低动物死亡率，根据大鼠体重选择相应直径的栓线可提高造模的成功率。