HPLC法测定喉咽清口服液中齐墩果酸的含量

摘要

Objective To establish a method for the determination of oleanolic acid in Houyanqing Oral Liquids after being acid hydrolysis by HPLC. Methods The sample was hydrolyzed with hydrochloric acid- ethanol for 1 hours and extracted with petroleum ether (60-90℃);the HPLC method was adopted. The Ultimate XB-C18 column (4.6 mmí250 mm, 5μm) was used at the column temperature of 30℃. The mobile phase was composed of acetonitrile-water (90:10) at a flow rate of 1.0 mL/min, the detected wavelength was set at 210 nm. Results The linear ranges of oleanolic acid was 2-12 μg, the average recovery rate was 99.09% with the RSD was 1.83%. Conclusion The developed method is so con-venient and has better precision and repeatability. This method can be used to determine the content of oleanolic acid in Houyanqing Oral Liquids.%目的：建立HPLC-UV法测定酸水解后喉咽清口服液中的齐墩果酸的含量。方法以盐酸-乙醇溶液为水解溶剂，水浴加热回流1 h，再用石油醚(60~90℃)萃取得齐墩果酸；采用高效液相色谱法，以Ultimate XB-C18(4.6 mm×250 mm，5μm)色谱柱，乙腈-水(90：10)作为流动相，流速为1.0 mL/min，柱温为30℃，检测波长为210 nm。结果齐墩果酸线性范围为2~12μg，平均回收率99.09%，RSD为1.83%。结论所建立方法操作简便、准确、可靠、重现性好，可用于喉咽清口服液中齐墩果酸的含量测定。