一氧化氮合成酶对豚鼠形觉剥夺性近视抑制作用研究

摘要

目的 观察一氧化氮对豚鼠形觉剥夺性(FDM)近视的影响,探讨一氧化氮对近视视网膜的调控作用.方法 以豚鼠作为实验材料,建立FDM模型,分为A组(无FDM处理)、B组(FDM诱导10d)、C组(FDM诱导20 d)、D组(FDM诱导10 d+NOS抑制剂注射)、E组(FDM诱导20 d+NOS抑制剂注射)、F组(FDM诱导10 d+褪黑素注射)和G组(FDM诱导20 d+褪黑素注射),每组各15只.检测各组的屈光度、眼轴长度和视网膜组织中诱导型一氧化氮合成酶(iNOS)表达水平.结果 与A组相比,随着FDM诱导时间的延长,近视程度明显加深,眼轴明显延长,同时iNOS水平上升,差异有统计学意义(P＜0.05).与FDM诱导相比,使用NOS抑制剂处理可以降低近视程度,抑制眼轴增长,降低iNOS的水平(P＜0.05).结论 FDM豚鼠视网膜iNOS表达水平上升,而NOS抑制剂处理能够有效抑制近视的发生.因此抑制一氧化氮的合成降低了豚鼠形觉剥夺性近视发生.%Objective To observe the effect of nitric oxide on formation of deprivation myopia (FDM) in guinea pig,in order to investigate the regulation of nitric oxide on retinopathy of myopia.Methods Guinea pigs were used to establish the FDM model.They were divided into group A (FDM-untreated),group B (FDM induced 10-day),group C (FDM induced 20-day),group D (FDM induced 10-day plus NOS inhibitor injected),group E (FDM induced 20-day plus NOS inhibitor injected),group F (FDM induced 10-day plus melatonin injected),group G (FDM induced 20-day plus melatonin injected),with 15 guinea pigs in each group.The myopic refraction,axial length in each group and expression level of iNOS in the retina of guinea pigs were observed.Results Compared to group A,myopia increased and the axial length prolonged significantly,and the level of iNOS increased obviously in FDM-induced group,with statistically significant difference (P ＜ 0.05).Compared to FDM-induced group,the level of iNOS significantly reduced (P ＜ 0.05),and the degree of myopia and the axial length were severely inhibited in guinea pigs treated with FDM plus NOS inhibitor injected.Conclusion The expression of iNOS in retina in FDM guinea pigs increases,while NOS inhibitor can effectively inhibite the occurrence of myopia.The synthesis of nitric oxide promotes FDM in guinea pig.