目的 探讨长期低剂量金雀异黄素(Gen)干预对雄性子代大鼠肥胖的影响.方法 36只SPF级性成熟雌性大鼠,受孕后随机分为3组,分别为两种不同剂量的Gen组和对照组,每组12只.Gen剂量分别为每千克饲料中添加900 mg(G900组)和300 mg(G300组),对照组不添加Gen.子鼠于12周龄处死.实验期间每周观察雄性子鼠的体重生长情况;实验结束后留取脂肪组织,计算脂肪脏器系数,分离血清进行血脂、血糖、胰岛素和炎症因子的检测.结果 10～12周,与对照组比较,G300组体重明显升高,差异有统计学意义(P＜0.05).同时,G300组睾周、肾周、总脂肪湿重以及脂肪脏器系数均明显升高,差异有统计学意义(P＜0.05);此外,G900组和G300组雄性子鼠血清中总三酰甘油和极低密度脂蛋白水平明显升高,差异有统计学意义(P＜ 0.05);G300组血糖和胰岛素水平明显升高,差异有统计学意义(P＜0.05).结论 长期金雀异黄素干预可以促进大鼠肥胖的发生.%Objective To explore the effect of long-term low dose maternal genistein (Gen) intake leading to male offspring rats fat.Methods 36 sexual maturity female rats were randomized divided into 3 groups (12 rats in each group) after fertilization,2 kinds of Gen doses groups and control group.The doses of Gen were 900 mg/kg diet (G900 group) and 300 mg/kg diet (G300 group) respectively,no Gen were added in control group.Offsprings were sacrificed at 12 weeks.The body weight of male offsprings were observed;fat were weighted and the ratio of fat/body weight were calculated after experiment;blood was separated to detect blood lipids,blood glucose,insulin and inflammatory factors.Results Compared with control group,body weight of G300 group was increased significantly from 10 weeks to 12 weeks,the differences were statistically significant (P ＜ 0.05).Meanwhile,the weight of around testicles,kindey,total fat weight and ratio of fat/body weight in G300 group were increased significantly compared with control group,the differences were statistically significant (P ＜ 0.05).Futhermore,TG and VLDL-C levels in G900 group and G300 group were increased significantly compared with control group,the differences were statistically significant (P ＜ 0.05).B1ood glucose and insulin levels in G300 group were increased markedly compared with control group,the differences were statistically significant (P ＜ 0.05).Conclusion Long-term genistein intake can promote offspring rats fat.
展开▼
