p-CHEK2在浸润性乳腺癌中的表达和临床意义

摘要

Objective To detect protein expression of phosphorylated chekpoint kinase 2 (P-CHEK2Thr68) in invasive breast cancer tissues and reveal the relevant clinical significance.Methods Totally 289 cases of invasive ductal breast cancer tissues were collected from January 2007 to December 2011 in Changhai Hospital Affiliated to Second Military Medical University and Shanghai Huangpu District Central Hospital.The expression of p-CHEK2Thr68 was analyzed by immunohistochemistry,and its relationship with clinicopathological parameters was analyzed.Results The positive expression rate of p-CHEK2Thr68 in breast cancer tissues was 26.0％ (75/289),the positive expression rate of the adjacent tissues was 0％,the difference was statistically significant (P ＜ 0.05).P-CHEK2Thr68 expression rate in the non-lymph node metastasis group was higher than the lymph node metastasis group,the ER negative group was higher than the ER positive group,the PR negative group was higher than the PR positive group,the HER2 negative group was higher than the HER2 positive group.P-CHEK2Thr68 expression rate in the tri-negative breast cancer group was higher than non-trinegative breast cancer group,the difference was statistically significant (P ＜ 0.05).There was no relationships between p-CHEK2Thr68 expression and the age,menstrual status,histological grade,pathological type,tumor size,TNM stage and p53 status.Conclusion Detection of p-CHEK2Thr68 protein expression by immunohistochemistry may help to investigate the biological behavior of invasive breast cancer and to develop individualized treatment strategy.%目的 研究在浸润性乳腺癌组织中磷酸化细胞周期检查点激酶2(p-CHEK2Thr68)的表达情况,并探讨其临床意义.方法 收集2007年1月～2011年12月第二军医大学附属长海医院及黄浦区中心医院289例乳腺癌改良根治术后病理标本.采用免疫组化方法检测组织中p-CHEK2Thr68的表达情况,分析其与临床病理参数之间的关系.结果 在乳腺癌组织中p-CHEK2Thr68阳性表达率为26.0％(75/289),癌旁组织的阳性表达率为0％,差异有统计学意义(P＜0.05).p-CHEK2Thr68表达率在无淋巴结转移组高于有淋巴结转移组,在ER阴性组高于ER阳性组,PR阴性组高于PR阳性组,HER2阴性组高于HER2阳性组,三阴性乳腺癌组高于非三阴性乳腺癌组,差异有统计学意义(P＜0.05),而p-CHEK2THr68的表达与患者的发病年龄、月经状态、组织分级、肿瘤大小、TNM分期、P53状态无关(P＞0.05).结论 采用免疫组化方法检测乳腺癌组织中p-CHEK2Thr68的表达情况以观察其生物学行为,将有助于制订乳腺癌患者的个体化治疗方案.