This paper introduces an enhanced type of hydrazine hydrate organic molecular fluorescence probe of high sensitivity and selectivity for biological monitoring. Its mechanism of action is based on the N2H4-triggered cleavage of the acetyl moiety to give 7-hydroxy-4-methylcoumarin. The properties of probe were characterized by means of spectroscopical test and fluorescent cell imaging. Results show that after adding N2H4 to the buffer solution, a significant fluorescence enhancement peak (33 times) appears at 451 nm of the fluorescence spectrum of probe solution. The quantitative analysis of detection limit is 9伊10-8 mol/L (y=20.3167+25.1778x, r=0.9996). The probe demonstrates high selectivity and specificity for N2H4 compared with other test samples(FN2H4=787,Fothers=22-24). In addition,the intracellular fluorescence imaging experiment shows that the fluorescence probe has the potential for N2H4 detection in vivo.%为合成适用于生物监测的水合肼荧光探针,该文基于水合肼诱导乙酰基脱保护生成7-羟基-4-甲基香豆素的原理,设计合成一种具有高灵敏度、高选择性的水合肼荧光增强型有机分子探针.采用光谱学测试及细胞荧光成像的方法,对探针的性能进行表征.实验结果表明:在缓冲溶液中,加入N2H4后,探针溶液的荧光光谱在451 nm处产生一个显著的荧光增强峰(33倍),定量分析检测限为9×10-8 mol/L(y=20.3167+25.1778x,r=0.9996),与其他测试物相比,探针表现出对N2H4较高的选择性和专一性(F水合肼=787,F其他=22~24);此外,细胞内的荧光成像实验,证明该荧光探针具有潜在检测细胞内N2H4的能力.
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