Objective:To explore the influence of acupuncture treatment on proinflammatory cytokines of osteoarthritic (OA) cartilage. Methods:Forty female SD rats were randomly and averagely divided into four groups:the normal,model, acupuncture and control group. The method of heel tendon resection for unilateral hind limb was used to establish OA animal models-that was to cut off the rat's heel tendon of left hind limb. The acupuncture group and control group were respectively treated by electro-acupuncture and Diclofenac on right hind limb of each animal for 2 weeks. Immunohistochemical staining was used to observe the expression characteristic of IL-ip and TNF-a in cartilage of each group,and the differences were compared among the groups. Results:Both IL-1βand TNF-a expression in model group were significantly up-regulated than those in the normal group(P<0.01). Their expression in model,acupuncture and control group were significantly different(P< 0.01). IL-lβ and TNF-a expression were significantly down-regulated in acupuncture and control group than that in model group (P<0.01),while the difference between the two former groups was not significant (P>0.05). Conclusion:Acupuncture can down-regulate the expression of IL-1β and TNF-a in osteoarthritic chondrocytes,and the regulating effect is the same as Diclofenac. This shows that acupuncture has a certain protective effect on osteoarthritic cartilage.%目的:探讨针刺对骨关节炎(OA)软骨中炎性细胞因子的影响作用.方法:选用SD雌性大鼠40只,采用随机数字表,随机分为正常组、模型组、针刺组和对照组,各10只.采用单侧后肢跟腱切除法建立OA动物模型,切除左后肢跟腱,分别采用电针和扶他林乳剂对针剌组和对照组非手术侧(右后肢)进行治疗.采用免疫组化技术,观察各组关节软骨中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-(α)表达的特点,并比较各组间的差异.结果:模型组IL-1β和TNF-α的表达都较正常组上调(P<0.01);模型组、针刺组和对照组3组比较,IL-1β和TNF-α的表达差异有统计学意义,针剌组和对照组的表达均较模型组下调(P<0.01),两者在针刺组与对照组的表达差异无统计学意义(P>0.05).结论:针剌能下调OA软骨细胞IL-1β、TNF-α的表达,与扶他林乳剂作用相当,说明针剌对OA软骨具有一定的保护作用.
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