目的了解肝癌患者乙型肝炎病毒(HBV)DNA含量及其与C基因启动子(BCP)基因变异的关系.方法采用PCR荧光实时定量技术检测114例肝癌患者及100例非肝癌乙肝患者HBV DNA含量.采用PCR-微板核酸分子杂交ELISA技术对肝癌及乙肝患者进行BCP基因变异检测.结果肝癌患者48%HBV DNA阳性,平均拷贝量为4.7×106拷贝/ml.BCP区域1762、1764位突变率为27%,且BCP变异的患者HBV拷贝量明显大于非变异患者.100例非肝癌乙肝患者HBV DNA阳性率41%,平均拷贝量3.8×105拷贝/ml,BCP基因突变率为8%,肝癌患者的BCP基因突变率明显高于乙肝患者.结论HBV感染可能是导致肝癌发生的重要原因,HBV BCP变异可能与病变程度有关.%Objective: To understand the relationship between HBV-DNA concentration and BCP gene mutation of hepatocarcinoma patients. Methods: FQ- PCR method was applied to detect HBV- DNA concentration in 114 hepatocarcinomas and 100 hepatitis B patients, PCR- microplate nucletide aid hybridization- ELISA technique was applied in the analysis of BCP gene mutation. Results: The HBV- DNA positive rate of hepatocarcinoma patients was 48 %, the average HBV - DNA concentration was 4.7 × 106 copies/mi. The BCP gene mutation rate was 27%, HBV - DNA concentrations in BCP mutation samples were significantly higher than in non - mutation samples. The HBV - DNA positive rate of hepatitis B patients was 41%, the average HBV - DNA concentration was 3.8 × 105 copies/ml. The BCP gene mutation rate was 8%. The BCP gene mutation rate of hepatocarcinoma patients was remarkably higher than hepatitis B patients. Conclusions: HBV infection is an important factor leading to hepatocarcinoma and HBV BCP mutation is related to symptoms degree.
展开▼
