目的：：观察接种不同代数的菌株及不同药物剂量组治疗后的梅毒兔主要脏器的病理改变。方法：睾丸接种组选择原代组、一代组、二代组及阴性对照组各1只进行解剖,取心、肝、脾、肾。背部皮肤的接种组药效学评价选择阳性对照家兔1只、普鲁卡因青霉素和头孢曲松中间两个剂量组治疗的家兔各1只,解剖心、肝、脾、肾,10％福尔马林溶液固定、包埋、切片和HE染色。结果：梅毒螺旋体感染家兔后2周,可引起心、肝和肾炎症细胞的浸润,但不引起脾脏的病理改变。一代组及二代组引起脏器的炎症改变与原代组相似。梅毒感染后的家兔药物治疗后12周,部分梅毒兔主要脏器仍有炎症反应,头孢曲松治疗RPR滴度转阴11周后家兔的心、肝及肾脏无明显炎细胞浸润。结论：在进行梅毒兔药效学模型建立过程中无需增加病理学的数据,但在治愈转阴后通过病理切片的观察能够更好地了解治疗效果。%Objective:To investigate the pathological changes of major organs in rabbit models inoculated with Treponema pallidum which has different continuous passages and is treated with different drugs and doses. Methods:One New Zealand rabbit was selected to bedissected. The heart, liver, spleen and kidney from pri-mary, first and second generation group and negative control group in testis-inoculated groups were used for the pathological examination. In dorsal skin-inoculated groups, these organs were taken from one rabbit select-ed from positive control group, two doses of procaine benzylpenicillin and ceftriaxone sodium groups. After fixed by 10% formalin solution, these organs were dehydrated, paraffine-embedded and sliced for HE stai-ning. Results:After infected with Treponema pallidum for two weeks, the heart, liver, and kidney tissue of the rabbit were infiltrated within flammatory cells, but no pathological changes were observed in spleen tissue. And also there were no significant difference in the inflammatory changes of the organs between the three pas-sages of Treponema pallidum. After treated with drugs for 12 weeks, some major organs of the rabbit were infil-trated with inflammatory cells, but no obvious infiltration of inflammatory cells were observed in these tissue of the infected rabbit treated with ceftriaxone sodium after the RPR titer was negative for 11 weeks. Conclusion:Pathologic observation is not essential for pharmacological evaluation, but the results can verify the efficacy of the treatment.
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