AIDS患者巨噬细胞对白念珠菌的吞噬和灭活功能研究

摘要

目的:明确AIDS患者巨噬细胞对白念珠菌的吞噬和杀灭活性功能.方法:提取AIDS患者和健康者的单核细胞,诱导分化为巨噬细胞.荧光显微镜和流式细胞仪测定巨噬细胞对白念珠菌的吞噬活性;CFU计数法分析巨噬细胞对白念珠菌的杀灭活性;测定巨噬细胞内亚硝酸盐含量,分析呼吸爆炸相关产物一氧化氮的生成;Western blot法测定巨噬细胞磷酸化酪氨酸激酶的表达.结果:AIDS患者的巨噬细胞对白念珠菌的吞噬、杀灭能力、一氧化氮及磷酸化Syk的蛋白均低于健康者.结论:AIDS患者巨噬细胞吞噬和杀灭白念珠菌活性降低.%Objective:To determine the phagocytosis and deactivation of macrophages to Candida albicansin in AIDS patients.Methods:The monocytes from AIDS patients and healthy controls were isolated, and then, were induced to macrophages in vitro.Phagocytosis and activity of macrophages to Candida albicans was detected by fluorescence microscope and flow cytometry and eactivation activity was detected by CFU counting.The level of NO was assessed according to the content of nitrite inside macrophages.The level of phosphorylated spleen tyrosine kinase was measured by Western blot.Results:The phagocytosis and deactivation of macrophages to Candida albicansin and the levels of NO and phosphorylated spleen tyrosine kinase in AIDS patients were lower than those in healthy controls.Conclusion:The phagocytosis and deactivation of macrophages to Candida albicansin in AIDS patients were inhibited.