仙珍骨康胶囊对小鼠地塞米松骨质疏松模型关键疗效指标的影响

摘要

目的: 明确仙珍骨康胶囊对小鼠地塞米松骨质疏松模型关键疗效指标的影响.方法: 40只C57BL/6小鼠随机分为A组:臀肌注射地塞米松磷酸钠,每周2次;B组:仙珍骨康混悬液20 mL/kg灌胃,每日1次;C组:地塞米松+仙珍骨康组;D组:空白对照组.实验6周后,处死全部老鼠.多模式活体成像系统、透射电镜检测各组小鼠骨密度及成骨细胞形态,检测各组小鼠血清中血钙、血清无机磷(Pi)、甲状旁腺激素(PTH)、I型前胶原C-端肽(PICP)、骨钙素(BGP)、骨碱性磷酸酶(BALP)、雌二醇(E2)、孕酮(PROG)及降钙素(CT)的水平.结果: A组骨密度与D组相比明显下降(P<0.05),C组骨密度与D组相比差异无统计学意义(P>0.05).透射电镜检测发现A组成骨细胞溶解;B组成骨细胞结构正常;C组成骨细胞核为卵圆形,有胶原分泌.A、B、C、D组PTH、PICP、BGP、BALP、E2、PROG、CT水平比较,差异均有统计学意义(均P<0.05).各组血钙及Pi水平的差异均无统计学意义(均P>0.05).结论: 仙珍骨康胶囊可能通过上调PTH、PICP、BALP,下调BGP、E2、CT与PROG水平来促进骨合成并增加骨密度.%Objective: To determine the effect of Xianzhengukang capsule on the glucocorticoid-induced osteoporosis. Methods:Forty C57BL/6 mice were randomly divided into four groups,group A:gluteal muscle injection of dexamethasone sodium phosphate twice a week; group B: Xianzhengukang mixed suspension 20 mL/kg,once a day;group C:dexamethasone + Xianzhengukang and blank control group (group D). Bone mineral density and osteoblast shape were detected by multi-mode living imaging system and transmission e-lectron microscopy (Tem). The levels of blood calcium, phosphorus, parathyroid hormone (PTH), type I collagen in mice serum C-peptide(PICP),osteocalcin(BGP),bone alkaline phosphatase(BALP),estra-diol(E2),calcitonin (CT) and, progesterone (PROG) were detected. Results: The level of bone mineral density in the group A was lower than that in the group D (P<0.05) and there was no significant difference between the group C and group D(P>0.05).The osteoblast was dissolved in group A and was normal in group B. The osteoblast nuclei were ovoid and secreted collagen in group C. There were significant differences in the levels of PTH,PICP,BGP,BALP,E2,PROG and CT between the group A,B,C and D (Ps<0.05) and there were no significant in the level of serum calcium and CT(Ps>0.05).Conclusion:Xianzhengukang cap-sule can promote bone formation and increased bone mineral density,which may be through up-regulating the levels of PTH,PICP,BALP and down-regulating the levels of BGP,E2,CT and PROG.