cyclinD1及p21WAF1表达与大肠癌关系研究

摘要

目的：探讨cyclinD1及p21WAF1表达与大肠癌发生发展的关系。方法：用免疫组化SP法对40例大肠癌标本进行cyclinD1和p21WAF1蛋白表达的检测，并与同期28例大肠腺瘤、14例正常大肠粘膜作对比。结果：cyclinD1和p21WAF1的阳性表达率在大肠腺瘤(92.9%)和大肠癌(67.5%)均显著高于正常大肠粘膜(35.7%)(P＜0.01，P＜0.05)。cyclinD1的表达与大肠癌的分化程度及淋巴结转移无关。p21WAF1的阳性表达率在大肠癌(37.5%)显著低于正常大肠粘膜(92.8%)(P＜0.01)。P21WAF1表达与大肠癌的分化程度及淋巴结转移有关。结论：cyclinD1和p21WAF1表达异常在大肠癌的发生、发展中是一个普遍事件。%Objective:To investigate expression of cyclinD1 and p21WAF1 and their relationship with multistage process of colon carcinogenesis.Methods:Immunohistochemical staining was performed with SP assay on archival materials from 40 colorectal carcinomas,28 colorectal adenomas,and 14 normal mucosa,cyclinD1 and p21WAF1 expression were compared.Results:The positive staining rate for cyclinD1 in colorectal adenomas and carcinomas is 92.9% and 67.5% respectively,which are both higher than that in the normal mucosa (35.7%) (Padenoma＜0.01，Pcarcinoma＜0.05) and the expression of cyclinD1 is not correlate with differentiation and lymph node metastasis of colorectal carcinoma.The positive stainimg rate for p21WAF1 in normal mucosa (92.9%) is higher than that in colorectal carcinoma(37.5%) (P＜0.01) and the expression of p21WAF1 is correlate with differentiation and lymph node metastasis of colorectal carcinonma.Conclusions:The aberrant expression of cyclinD1 and p21WAF1 is a common event during multistage process of colon carcinogenesis.