安徽地区羊口疮病毒VIR基因PCR检测及遗传进化分析

摘要

采集安徽某地区两个山羊场中疑似感染ORFV的羔羊唇部结痂病料,采用PCR方法对毒力基因VIR进行了扩增,并获得了目的片段VIR-1和VIR-2.同时将PCR产物克隆至pMD18-T载体,鉴定后测序.序列分析结果表明,两个羊场的毒力基因VIR-1和VIR-2基因片段与参考毒株的相关基因核苷酸同源性分别为95.8％～100％和95.4％～99.6％.基因进化树比较显示,两分离株分别与美国AY424975.1株和甘肃KF916586.1株的亲缘关系最近.%The kid lacrimal scabs were collected from two farms in Anhui Province for amplifying the VIR gene by PCR method.Successfully,the target gene were got,and named VIR-1,and VIR-2,respectively.Then,the PCR product was inserted into pMD18-T Vector,then identified and sequenced.The sequence analysis showed that the nucleotide sequence homology of VIR-1and the referenced gene was 95.8％-100％,while the nucleotide sequence homology of VIR-2 and the referenced gene was 95.4％-99.6％.The results of phylogenetic tree showed that they were the closest to AY424975.1 strain and KF916586.1 strain respectively.