2型糖尿病患者抗心磷脂抗体水平与并发症发生率的临床研究

摘要

目的：观察2型糖尿病患者血清中抗心磷脂抗体（ACL）水平与糖尿病相关并发症发生率的相关性。方法收集2014年6月～2015年6月就诊于我院的2型糖尿病患者180例（平均年龄44±17岁），其中合并血管并发症患者为A组（112例），未合并血管并发症患者为B组（68例），健康对照组为C组（180例，平均年龄41±19岁），记录所有入组个体的基本资料，检测空腹血糖，血脂，糖化血红蛋白，24 h尿蛋白，尿蛋白/肌酐，肾小球率过滤（GFR）。采用酶联免疫吸附法测定所有入组个体血清中抗心磷脂抗体（ACL，IgG及IgM亚型）的水平。结果 A、B两组患者血清中IgG ACL及IgM ACL抗体的水平高于C组（P＜0.001），且A组患者IgM ACL水平高于B组及C组（P＜0.05），但A、B组间IgG ACL水平无差异。结论抗心磷脂抗体水平在合并血管并发症的糖尿病患者中增高，表明ACL可能参与了2型糖尿病的血管并发症的发生。%Objective To observe the prevalence of anticardiolipin antibodies(ACL)antibodies in type 2 diabetes patients with and without vascular complications.Methods 180 adults with type 2 diabetes melitus patients were enroled in this study,including 118 subjects with vascular complications (group A)and 68 subjects without vascular complications(group B),the control group(group C)was composed of 180 age and sex-matched healthy individuals. The basic information of each subject was recorded. Fasting blood glucose,blood lipid levels,HbA1c,Estimated GFR, albuminuria,albumin-to-creatinine ratio and ophthalmologists of each subject were measured. The levels of ACL immunoglobulin IgG and ACL IgM antibodies were measured by enzyme linked immunosorbent assays.Results Compared with group C,anti-cardiolipin antibodies of IgG and IgM were significantly increased in in group A and B(P<0.001),and IgM aCL in group A was higher than group B and C(P<0.05),but the level of IgG ACL was no difference between patients with and without vascular complications(P>0.05).ConclusionThe higher prevalence and levels of anti-cardiolipin antibodies in patients with vascular complications suggests that these humoral factors might be involved in the vascular complications of type 2 diabetes melitus.