目的：观察花姜酮处理对鼻咽癌CNE-2细胞放疗敏感性的影响。方法体外培养鼻咽癌细胞系CNE-2。并根据不同的实验目的分为对照组、γ-射线干预组以及γ-射线联合花姜酮处理组。其中对照组包括空白对照组和花姜酮处理组,分别给予等体积培养基或0~20μmol/L花姜酮处理48 h,γ-射线组采用剂量率为100cGy的γ-射线照射,联合治疗组细胞采用花姜酮预处理24 h后用γ-射线照射。处理结束后采用MTT法检测CNE-2细胞增殖情况,流式细胞术检测CNE-2细胞周期改变情况。结果0~20μmol/L花姜酮或100cGy γ-射线单独处理48 h后,对人鼻咽癌CNE-2细胞生长无明显影响；而同时联合不同浓度花姜酮与100cGy的γ-射线处理后,随着花姜酮浓度的增高,细胞增殖水平随之降低。流式细胞术也显示,0~20μmol/L花姜酮联合100cGyγ-射线照射后,可将细胞阻滞在G1期,同时可降低S期、G2/M期细胞的比例。结论花姜酮通过改变CNE-2细胞周期而发挥对细胞放疗增敏作用。%Objective To observe the effect of zerumbone treatment on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cells. Methods nasopharyngeal cancer cell line CNE-2 was cultured in vitro and divided into the control group, gamma ray intervention group and gamma ray combined with zerumbone treatment group, among them, the control group in-cluded the blank control group and the zerumbone treatment group, and they were respectively given equal-volume culture media or 0-20μmol/L zerumbone treatment for 48h, the gamma ray group adopted 100cGy gamma ray radiation, the com-bined treatment group adopted gamma ray radiation after the zerumbone pretreatment for 24h, and the CNE-2 cell prolifer-ation was detected by MTT method and CNE-2 cell cycle changes were detected by flow cytometry at the end of treatment. Results After 48h single treatment, 0-20μmol/L zerumbone or 100cGy gamma ray radiation had no obvious effect on the human nasopharyngeal carcinoma CNE-2 cell growth; but the combined treatment of different concentrations of zerumbone and 100cGy gamma ray showed that with the increase of the zerumbone concentration, the cell proliferative level decreased, and the flow cytometry also showed that after the treatment of 0-20μmol/L zerumbone and 100cGy gamma ray radiation, the cells were arrested in G1 phase, at the same time, the cell proportions in S stage and G2/M stage could be reduced. Con-clusion Zerumbone plays a sensibilization role in cell radiotherapy by changing the CNE-2 cycle.
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