sumo－1在大鼠肝部分切除后再生过程中表达变化的实验研究

摘要

Objective:By establishing the model of rat liver resection,to study relationship between sumo-1 and liver regeneration.Method:Choose healthy clean level 80 male Sprague-Dawley rats weight 200-220g.80 rats were randomly divided into 2 groups,control group (group A),the experimental group (group B),each group 40,each group randomly divided into 4 groups,each group of 10.The experimental group and control group were given 70% liver resection surgery,postoperative experimental postoperative subcutaneous injection HGF (100 mg/kg/d),the control subcutane-ous injection the same amount of normal saline until death.A,B group of rats respectively after 1,3,5,and 7 days in the ether anesthesia executed only 10,get the liver and liver regeneration rate calculation,PCNA immunohistochemical detection of liver at each time point,take 2 ml,inferior vena venous blood biochemical analysis of AST,ALT,Postoperative detected using RT-PCR HGF and express the sumo-1.Result:Groups of liver regen-eration rate:group B at the same time point regeneration rate is significantly greater than in group A (P < 0.05);Group B is bigger than the PCNA in group A (P < 0.05);B group of AST,ALT are much smaller than A point in time in group A (P < 0.05);Group B HGFmRNA at each time point, the sumo-1 mrna expression was significantly higher in group A (P < 0.05).Conclusion:sumo-1 expression is associated with liver regeneration,as liver regeneration increases,the sumo-1 expression increases .sumo-1 expression is associated with liver regeneration,as liver regeneration increases, the sumo-1 expression increases.%目的：：通过建立大鼠肝切除模型,研究 sumo－1与肝再生的关系.方法：选用健康清洁级雄性SD大鼠80只体重200－220g.将80只大鼠随机分成2组,对照组(A组),实验组(B组)每组40只,每组各随机分成4组,每组10只.实验组和对照组均行70％肝切除手术,术后实验组术后皮下注射 HGF (100mg/kg/d),对照组皮下注射等量生理盐水直至处死.A 、B 组大鼠分别术后1、3、5、7天在乙醚麻醉下各处死10只,取其肝脏并计算肝再生率,各时间点免疫组化检测肝脏PCNA,取下腔静脉血2ml,生化分析AST,ALT；术后用RT－PCR检测 HGF和sumo－1表达.结果：各组肝再生率：B组在各相同时间点再生率明显大于A组(P＜0．05)；B组PCNA明显大于A组(P＜0．05)；B组AST,ALT明显小于各时间点A组(P＜0．05)；B组各时间点 HGFmRNA、sumo－1mRNA表达明显高于 A组(P＜0．05),结论：sumo－1表达与肝再生有关,随着肝再生增强,sumo－1表达增加.