绿宝石梨组织快繁技术的研究

摘要

本研究以优良品种绿宝石梨的嫩茎稍为试材，对影响组织培养的主要因素，包括培养基种类、生长调节剂浓度配比进行了研究，建立了绿宝石梨的组织快繁技术。试验结果表明，适宜绿宝石梨组织培养的启动培养基由MS、1.0mg/L 6-BA、0.1mg/L IBA组成；增殖培养基是由1/2MS、1.0mg/L 6-BA、0.1mg/L IBA组成。%In this paper, tender stems of Lvbaoshi pear were used as test material. Effects of media types, 6-BA and IBA with different concentrations on Lvbaoshi pear issue culture were studied, which established the fast-breeding technique of Lvbaoshi pear. The results showed that the suitable culture medium for different stages were initial medium and multiplication medium. Initial medium included MS, 1.0mg/L 6-BA, and 0.1mg/L IBA. Multiplication medium included 1/2MS, 1.0mg/L 6-BA, and IBA 0.1mg/L.