目的：研究植物雌激素对糖尿病大鼠周围神经病变的影响，探讨植物雌激素治疗糖尿病周围神经病变的可行性及疗效。方法①建立大鼠动物模型。②药物干预：植物雌激素治疗组、阴性对照组、糖尿病组分别给予金雀异黄素、神经营养因子、生理盐水肌注。③标本取材与制备：取大鼠胫神经切片光镜和电镜下观察。④图像分析：光镜切片得到有髓纤维的髓鞘和轴突面积，神经内外膜的血管数。电镜切片观察有髓神经纤维和神经营养血管内皮的改变。结果糖尿病组胫神经的有髓神经纤维和神经营养血管组织形态学出现明显变化、阴性对照组正常，植物雌激素治疗组有轻微变化。结论植物雌激素治疗糖尿病周围神经病变是可行和有疗效的。%Objective To study the effect of phytoestrogen on diabetic peripheral neuropathy in rats, and investigate the feasibility and effect of phytoestrogen in the treatment of diabetic peripheral neuropathy. Methods①A rat animal model was established②Drug intervention: Phytoestrogen treatment group, negative control group, diabetes group was treated with intramuscular injection of genistein, neurotrophic factor, physiological saline, respectively.③Sample collection and preparation: Rat tibial nerve sections were collected and observed under light microscope and electron microscope.④Image analysis: Light microscopic sections obtained by myelinated fiber myelin and axon area, the number of inner and outer membrane of vascular nerve. The observation under electron microscope showed that there were changes in myelinated fibers and nerve nutritional vascular endothelium.⑤Statistical analysis: Morphological index of the image was analyzed statistically by variance analysis.Results The myelinated fibers and nerve nutritional vascular histomorphology of tibial nerve in the diabetic group appeared obvious changes, while those of the negative control group were normal, those of phytoestrogen treatment group had a slight change.Conclusion Phytoestrogen is feasible and effective in the treatment of diabetic peripheral neuropathy.
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