“米邦塔”食用仙人掌体外抗氧化活性的研究

摘要

The OMP extracts by different extracting solvents showed antioxidant activities in vitro were tested. Reducing power test, deoxidization Fe 3+ , anti - lipid peroxidation of linoleic acid and lecithin liposome ,1,1- diphenyl - 2 - picrylhydrazyl (DPPH) radical and superoxide radical scavenging assays, with butylated hydroxyanisole ( BHA) and ascorbic acid as positive controls. The results suggested that methanol OMP powder had strongest scavenging activities and deoxidization Fe3 + ; 30mg/mL OMP water extracts had strongest anti - lipid peroxidation of linoleic acid activities.%分别采用普鲁士兰法测定还原力、硫氰酸铁法测定亚油酸氧化抑制能力、比色法测定1,1-二苯基-2-苦基肼[DPPH·]自由基清除能力、化学发光法测定超氧阴离子自由基[O2-·]清除能力,对仙人掌不同溶剂提取物的抗氧化性进行体外试验评价.结果表明仙人掌皮粉甲醇提取物还原力最大,[ DPPH·]自由基和[O2-·]清除能力最高,其中仙人掌皮粉甲醇提取物在[DPPH·]自由基的半抑制率IC50为1.865mg/mL,在亚油酸氧化抑制能力中30mg/mL仙人掌水提物最强.