羧甲基壳聚糖硒对小鼠生长性能、血液硒含量及抗氧化功能的影响

摘要

This experiment was conducted to determine the effects of carboxymethyl chitosan selenium on growth per-formance,blood selenium content and antioxidation capacity in mice.One hundred and twenty KM mice,female and male in half,were randomly assigned into three dietary treatment groups for a 28 days study,which were fed basal diets,basal diets supplemented with sodium selenite and basal diets supplemented with carboxymethyl chitosan selenium ,respectively. The results showed that feed intake and feed/gain ratio(F/G)in T2 group were 5.24%,8.66%and 4.27%,4.29%lower than those in T1 group and C group,while there were no significant differences in feed intake,F/G and weight gains of mice a-mong T2 group,T1 group and C group(P > 0.05).Blood selenium contents and plasma total antioxidant capacity(T-AOC) levels in T2 group were 78.95%(P<0.01),17.50%(P<0.01)and 17.24%(P<0.05),8.05%(P<0.05)higher than those in C group and T1 group,blood selenium contents and plasma T-AOC levels in T1group were 52.63%(P < 0.05)and 8.75%(P<0.05)higher than those of C group.Blood glutathione peroxidase (GSH-Px)activities in T2 group and T1 group were 24.65%(P < 0.01)and 14.13%(P < 0.05)higher than that of C group.Blood GSH-Px activities in T2 group were 9.22% higher than that in T1 group (P > 0.05).Plasma malonaldehyde (MDA) contents in T2 group were 29.40%(P <0.01) and 18.66%(P < 0.05)lower than that in C group and T1 group.Plasma MDA contents in T1 group were 13.21%(P< 0.05)lower than that in C group .The results indicated that carboxymethyl-chitosan selenium supplementation could in-crease blood selenium content and antioxidant capacity in mice,and its effects were better than sodium selenite in mice. While carboxymethyl-chitosan selenium had no effect on growth performance in mice.%为研究羧甲基壳聚糖硒对小鼠生长性能、血液硒含量及抗氧化功能的影响，本试验将120只KM小鼠（雌雄各半），随机均分为C组、TI组和T2组，分别喂基础日粮、添加亚硒酸钠的基础日粮和添加羧甲基壳聚糖硒的基础日粮，试验持续28 d。结果表明：T2组小鼠采食量和料重比较C组和T1组分别降低了5.24%、8.66%和4.27%、4.29%；但T2组、T1组和C组间小鼠的采食量、料重比及增重均无显著差异（P＞0.05）。 T2组小鼠全血硒含量和血浆总抗氧化能力（T-AOC），较C组和T1组分别提高了78.95%（P ＜0.01）、17.50%（P ＜0.01）和17.24%（P ＜0.05）8.05%（P＜0.05）；T1组小鼠全血硒含量和血浆T-AOC较C组分别提高了52.63%（P＜0.05)和8.75%（P＜0.05）。 T2组和T1组小鼠全血谷胱甘肽过氧化物酶（GSH-Px）活性分别较C 组提高了24.65%（P ＜0.01）和14.13%（P ＜0.05）；T2组小鼠全血GSH-Px活性较T1组提高了9.22%，但未达差异显著水平（P＞0.05）。 T2组小鼠血浆丙二醛（MDA ）含量分别较C组和T1组降低了29.40%（P＜0.01）和18.66%（P＜0.05）；T1组小鼠血浆MDA 含量较C组降低了13.21%（P＜0.05）。结果提示：羧甲基壳聚糖硒能够提高小鼠血液硒含量和抗氧化能力，其效果优于亚硒酸钠；羧甲基壳聚糖硒对小鼠生长性能无影响。