A pilot-scale biological filter was constructed to removal of iron, manganese and high ammonia simultaneously from groundwater. The results indicated that the failure of manganese and ammonia oxidation when the influent ammonia concentration was above 2.0mg/L without extra aeration. Oxidation of iron, manganese and ammonia could be completed by downflow filtration with the aeration at the bottom of the filter bed, while the processing capacity was affected significantly and was not suitable for application of the biofilter. The upflow filtration with the same aeration mode could also met the oxygen demands of the oxidation process, however, the oxidized iron and manganese were easily taken out filter bed, causing high iron and manganese concentration in the effluent. For the upflow filtration with extra aeration at the bottom of the filter bed, the experiments demonstrated that the intercepting capacity of filter was damaged significantly by disturbance of aeration compared to the case of upflow filtration without aeration and was inversely correlatedwith the aeration intensity.%以中试有机玻璃滤柱反应器进行伴生高浓度氨氮含铁锰地下水生物同步净化试验研究，结果表明，进水氨氮浓度高于2.0mg/L 时，溶解氧浓度是限制锰与氨氮去除的主要原因.下向流底部曝气过滤运行(气水逆向)可解决溶解氧不足的问题，但滤柱滤水能力有限；上向流底部曝气过滤(气水同向)同样可以满足铁锰氨氮氧化需氧量，然而曝气扰动作用削弱了滤池过滤截留能力造成铁锰氧化物穿透滤层，致使滤层出水铁锰浓度无法满足标准要求，曝气量越大，其滤池截留能力越弱.
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