在调查夏季水华期间太湖梅梁湾和贡湖湾采样点水下光谱的基础上,设计不同色光(红光、蓝光、白光对照)的室内模拟实验,探究色光对微囊藻生长及群体维持的影响.结果显示,采样区域(梅梁湾 M1、M2和贡湖湾 G1、G2)背景浊度值较高,水下光谱偏向黄红光波段.室内模拟实验显示,微囊藻群体在不同色光下培养24d后,红光组群体粒径高于蓝光组和白光对照组.并且,红光有利于提高微囊藻群体多糖含量(总多糖和固着性胞外多糖).不同色光培养单细胞铜绿微囊藻(Microcystis aeruginosa FACHB-469)的结果显示,白光对照组的细胞浓度始终最高,其次是红光组,最后是蓝光组.野外监测和室内模拟实验表明,太湖夏季水华期间采样区域偏向黄红光波段的水下光谱有利于微囊藻群体粒径的维持和生长,这是野外群体微囊藻与室内单细胞微囊藻重要的生境差异,可能是微囊藻群体形成和维持的影响因素.%Indoor simulation experiments of different chromatic light (red light, blue light, and white light) were designed based on the underwater spectra of sampling sites at Meiliang Bay (M1, M2) and Gonghu Bay (G1, G2) during summer blooms, so as to investigate effects of chromatic light on the growth and colony maintenance of Microcystis. Results showed that the background turbidity at sampling sites was relatively high, and the underwater spectra shifted to the band of yellow and red light. Results of indoor simulation experiment showed that Microcystis colonies of red light group were larger than those of blue light group and white light control after 24days. Data showed that red light was favorable to increase the content of polysaccharides (total polysaccharides and binding extracellular polysaccharides). As for Microcystis aeruginosa unicells (FACHB-469), cell concentration of white light control kept the highest level, followed by the red light group, and then the blue light group. Based on the field observation and indoor simulation, the underwater spectra at sampling sites were favorable for maintaining of Microcystis colony size and growth rate, which may possibly result in the differences of habitat heterogeneity between outdoor colonial Microcystis and indoor unicellular Microcystis. Changes of underwater spectra can influence the formation and maintenance of Microcystis colonies.
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